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由来種
Streptococcus pyogenes
発現系
Escherichia coli
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アフィニティタグ
His-MBP Tag (N-term)
保存バッファー
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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純度
>98% as determined by SDS-PAGE analysis.
分子量
The protein has a calculated MW of 201 kDa.
The protein migrates about 180 kDa under reducing condition (SDS-PAGE analysis). -
エンドトキシン量
<0.1 EU per 1 μg of the protein by the LAL method.
形状
Lyophilized
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仕様
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背景
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背景
The enhanced specificity Cas9 protein from Streptococcus pyogenes (~160 KD), modified at sites K848A/K1003A/R1060A, exhibit substantially reduced off-target cleavage in cells. When combined with target RNAs, eSpCas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and help the development of genetically-modified animals via one-cell embryo injection.
同義語
CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9
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Uniprot ID
#BDU59491.1
配列ノート
Asp41-Asp1407
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使用説明
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溶解
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
配送
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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安定性および保存
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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画像
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レビュー
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論文
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データシート & ドキュメント
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免責事項:For Research Use or Further Manufacturing Only.