カタログ番号

LDG0002RG

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概要

説明

Protein glycosylation is a complex posttranslational modification that manipulates biological activity such as protein folding, intracellular trafficking, stability, and half-life, affecting protein function. Endoglycosidase H is a glycosidase that cleaves asparagine-linked oligomannose and hybrid, but not glycan complex, from N-linked glycoproteins. It hydrolyses the bond connecting the two N-acetylglucosamine residues comprising the chitobiose core, leaving an N-acetylglucosamine residue on the asparagine.

製品ノート

After thawing, the buffers may crystallize, which is a normal occurrence. Warm crystallized buffer until the salt crystals return to solution. Ensure that your components return to RT before use in the assay.
Please fine-tune the input sample volume to find the optimal condition for your assay.
Once optimize for the cleavage condition, the cleavage reactions can be scaled up to cleave a large amount of the target fusion protein.

コンポーネント

Package	Items	Quantity
10,000 U	Endoglycosidase H (Endo H) (500 U/μL)	1 vial (10,000 U)
	10× Glycoprotein Denaturing Buffer	1 vial (1 mL)
	10× Reaction buffer	1 vial (1 mL)
50,000 U	Endoglycosidase H (Endo H) (500 U/μL)	1 vial (50,000 U)
	10× Glycoprotein Denaturing Buffer	1 vial (1 mL)
	10× Reaction buffer	1 vial (1 mL)

10,000 U
Items
Endoglycosidase H (Endo H) (500 U/μL)
Quantity
1 vial (10,000 U)
Items
10× Glycoprotein Denaturing Buffer
Quantity
1 vial (1 mL)
Items
10× Reaction buffer
Quantity
1 vial (1 mL)
50,000 U
Items
Endoglycosidase H (Endo H) (500 U/μL)
Quantity
1 vial (50,000 U)
Items
10× Glycoprotein Denaturing Buffer
Quantity
1 vial (1 mL)
Items
10× Reaction buffer
Quantity
1 vial (1 mL)

仕様
- 発現系
  
  Escherichia coli
  
  濃度
  
  500 U/μL
- 保存バッファー
  
  Endoglycosidase H is supplied in 20 mM Tris-HCl, 50 mM NaCl and 5 mM EDTA, pH 7.5
  
  純度
  
  >95% as determined by SDS-PAGE analysis.
- 単位定義
  
  One unit of Endoglycosidase H cleaves > 95% of the carbohydrate from 10 μg of denatured RNase B in a total reaction volume of 10 μL at 37°C for 1 h.
  
  エンドトキシン量
  
  <1 EU per 1 μg of the protein by the LAL method.
- 形状
  
  Liquid
使用説明
- 配送
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  安定性および保存
  
  This product is stable after storage at:
  - -20°C for -80°C long-term storage under sterile conditions.
  Avoid repeated free-thaw cycles.
画像

Endo H cleaves high mannose and hybrid- type glycans.

The standard assay was performed by incubating 1 unit of Endo H and 10 µg of RNase B under the above conditions. SDS-PAGE analysis of RNase B digested with Endoglycosidase H.

SDS-PAGE analysis of recombinant Endoglycosidase H

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合計 (3)
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データシート & ドキュメント

データシート

MSDS

マニュアル

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