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Leadgene Leadgene
Diaphorase
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カタログ番号

LDG0021RG

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  • 概要

    説明

    Diaphorase, also known as NADH dehydrogenase or NAD(P)H oxidoreductase, is an essential enzyme involved in cellular redox reactions. It facilitates electron transfer from NADH or NADPH to various acceptors, playing a crucial role in the electron transport chain. This enzyme helps mitigate oxidative stress by reducing harmful oxidants and is key in regulating metabolic pathways by maintaining NAD+/NADH and NADP+/NADPH ratios. Additionally, diaphorase contributes to cellular signaling processes, influencing cell proliferation, differentiation, and apoptosis.

  • 仕様

    • 発現系

      Escherichia coli

      活性

      ≥500 U/ mg

    • 単位定義

      One unit causes decrease in DCPIP by one unit of absorbance (1.0) per minute under the following conditions：27 mM Tris-HCl pH 7.5, 0.2 mM NADH, 40 μM DCPIP and 33 μg/ mL BSA.

      形状

      Lyophilized (Yellow amorphous powder)

  • 使用説明

    • 溶解

      It is recommended to weigh and reconstitute 10 mg of lyophilized powder in 250 μL double-distilled water directly and incubate the solution for at least 10 mins to ensure sufficient re-dissolved.

      配送

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • 安定性および保存

      This product is stable at -20°C for long-term storage under sterile conditions.
      Avoid repeated free-thaw cycles.

  • 画像
    The effects of various chemicals on Diaphorase. The enzyme was incubated with the chemicals at 25°C for 1 hour.

    The effects of various chemicals on Diaphorase.
    The enzyme was incubated with the chemicals at 25°C for 1 hour.

    Temperature activity of Diaphorase. The enzyme reactions in 0.2 M Tris-HCl buffer, pH 7.5, were carried out under different temperature.

    Temperature activity of Diaphorase.
    The enzyme reactions in 0.2 M Tris-HCl buffer, pH 7.5, were carried out under different temperature.

    pH activity of Diaphorase. The buffer conditions with various pH values were used in the reaction at 25°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    pH activity of Diaphorase. The buffer conditions with various pH values were used in the reaction at 25°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    Thermal stability of Diaphorase. The enzyme powder was reconstituted by double-distilled water and treated with different temperature for 30 minutes. Final concentration: 48 U/ mL.

    Thermal stability of Diaphorase. The enzyme powder was reconstituted by double-distilled water and treated with different temperature for 30 minutes. Final concentration: 48 U/ mL.

    pH stability of Diaphorase. The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 3 hours at 30°C. pH 6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0, 0.1 M Carbonate-bicarbonate buffer.

    pH stability of Diaphorase. The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 3 hours at 30°C. pH 6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0, 0.1 M Carbonate-bicarbonate buffer.

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  • 論文

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  • データシート & ドキュメント
    データシート
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免責事項：For Research Use or Further Manufacturing Only.

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