dCas9-VP64

カタログ番号

LDG003POE

データシート

その他のドキュメント

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• 仕様

  • 由来種

    Streptococcus pyogenes

    発現系

    Escherichia coli

  • アフィニティタグ

    His Tag (C-term)

    保存バッファー

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • 純度

    >90% as determined by SDS-PAGE analysis.

    分子量

    The protein has a calculated MW of 169.29 kDa.
    The protein migrates as 130-180 kDa under reducing condition (SDS-PAGE analysis).

  • エンドトキシン量

    <0.1 EU per 1 μg of the protein by the LAL method.

    形状

    Lyophilized

• 背景

  • 背景

    dCas9-VP64 refers to the dCas9 protein (#PDB: 5Y36_A) fused with the VP64 transcription activation domain, which is used in CRISPR-based gene activation. Comprising a deactivated Cas9 (dCas9) protein and VP64 transcriptional activator, it precisely targets gene promoters, enhancing gene expression without altering the DNA sequence. dCas9-VP64 offers a versatile tool for studying gene function, disease mechanisms, and potential therapeutic interventions.
    dCas9 protein is an endonuclease-inactive version of Cas9 (CRISPR associated protein 9). dCas9 lacks endonuclease activity so that it binds but does not cleave cognate DNA, which can be used to localize transcriptional activators or repressors to specific DNA sequences in order to control transcription.

    同義語

    CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9

  • 配列ノート

    Met1-Asp1368

• 使用説明

  • 溶解

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • 安定性および保存

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• 画像

  SDS-PAGE analysis of recombinant dCas9–VP64.

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  合計   (1)

• レビュー

  

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.