Language
EN
DE
JA
TW
Leadgene Leadgene
D-3-Hydroxybutyrate Dehydrogenase (3-HBDH)
もっと見る
カタログ番号

LDG0022RG

その他のドキュメント
パッケージと価格を読み込み中...
COA 検索
  • 概要

    説明

    D-3-Hydroxybutyrate dehydrogenase (3-HBDH) is an enzyme crucial in the metabolism of ketone bodies, specifically involved in the conversion of D-3-hydroxybutyrate to acetoacetate during ketogenesis. 3-HBDH plays a vital role in energy production, particularly under conditions such as fasting or prolonged exercise when ketone bodies serve as alternative energy sources. This enzyme is essential for maintaining metabolic balance and is found in various tissues, including the liver and kidneys. Understanding 3-HBDH's function is significant for insights into metabolic disorders and energy metabolism regulation.

  • 仕様

    • 発現系

      Escherichia coli

      活性

      ≥300 U/ mg

    • 単位定義

      One unit causes the formation of one micromole of NADH per minute under the following conditions: 0.1 M Tris-HCl pH 8.5, 25 mM 3-Hydroxybutyrate, 1.8 mM NAD+.

      形状

      Lyophilized (White amorphous powder)

  • 使用説明

    • 溶解

      It is recommended to weigh and reconstitute 10 mg of lyophilized powder in 250 μL double-distilled water directly and incubate the solution for at least 10 mins to ensure sufficient re-dissolved.

      配送

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • 安定性および保存

      This product is stable at -20°C for long-term storage under sterile conditions.
      Avoid repeated free-thaw cycles.

  • 画像
    The effects of various chemicals on 3-HBDH. The enzyme was incubated with the chemicals at 25°C for 1 hour.

    The effects of various chemicals on 3-HBDH.
    The enzyme was incubated with the chemicals at 25°C for 1 hour.

    Temperature activity of 3-HBDH. The enzyme reactions in 0.1 M Tris-HCl buffer, pH 8.5, were carried out under different temperature.

    Temperature activity of 3-HBDH.
    The enzyme reactions in 0.1 M Tris-HCl buffer, pH 8.5, were carried out under different temperature.

    pH activity of 3-HBDH. The buffer conditions with various pH values were used in the reaction at 37°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    pH activity of 3-HBDH.
    The buffer conditions with various pH values were used in the reaction at 37°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    Thermal stability of 3-HBDH. The enzyme powder was reconstituted by double-distilled water and treated with different temperature for 15 minutes. Final concentration: 15.9 U/ mL.

    Thermal stability of 3-HBDH.
    The enzyme powder was reconstituted by double-distilled water and treated with different temperature for 15 minutes. Final concentration: 15.9 U/ mL.

    pH stability of 3-HBDH. The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 20 hours. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    pH stability of 3-HBDH.
    The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 20 hours. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    1
    /
    5
    合計   (5)
  • レビュー

    この製品についてのご経験を共有してください。下のリンクから投稿いただけます。

    レビューを投稿
  • 論文

    現在論文はありません。下のリンクからお知らせください。

    論文を投稿
  • データシート & ドキュメント
    データシート
    MSDS
    マニュアル
    1
    /
    1

免責事項：For Research Use or Further Manufacturing Only.

本サイトでは、ユーザー体験の向上およびお客様に合わせた広告の配信を目的として、Privacy Policy に記載のとおり Cookie を使用しております。「同意する」をクリックして同意のうえ続行してください。