カタログ番号

LDG0016YA

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概要

説明

Zika virus belongs to the family Flaviviridae, genus Flavivirus. Zika virus is transmitted primarily by mosquitos, but also can be transmitted through sexual, vertical, and blood transfusion. NS1 is a highly-conserved viral protein in flaviviruses. In infected patients, the concentration of NS1 protein in circulating blood is relatively high, and can be detected up to 5 days after the onset of illness.

製品ノート

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:500-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
仕様
- ホスト
  
  Mouse
  
  クローナリティ
  
  Monoclonal
- アイソタイプ
  
  IgG2a
  
  クローン名
  
  clone ZK243
- 免疫原
  
  Zika virus NS1
  
  反応性
  
  Zika virus
- アプリケーション
  
  ELISA, WB, IFA, FACS
  
  コンジュゲーション
  
  Unconjugated
- 濃度
  
  1 mg/mL
  
  保存バッファー
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- 特異性
  
  NS1
  
  形状
  
  Liquid
使用説明
- 配送
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  安定性および保存
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
画像

ELISA titration of Anti-ZIKV NS1 Antibody [Clone ZK243]
Titration curve of anti-ZIKV NS1 antibody in ELISA. Red: ZIKV NS1; Black: BSA (negative control).

Immunofluorescence analysis of Anti-ZIKV NS1 Antibody [Clone ZK243]
ZIKV NS1 mAb (1:400) were used for detecting ZIKV NS1 in IFA. Scale bar: 200 μm

FACS analysis of Anti-ZIKV NS1 Antibody [Clone ZK243]
ZIKV or DENV-infected Vero cells were stained with mouse anti-ZIKV NS1 monoclonal antibody at 5 μg/mL (red) and without antibody control (black).

ELISA assay of Anti-ZIKV NS1 Antibody [Clone ZK243]
Indirect ELISA analysis was performed by coating a plate with recombinant NS1 protein of ZIKV, CHIKV or DENV and probing with anti-ZIKV NS1 Antibody.

Western blotting analysis of Anti-ZIKV NS1 Antibody [Clone ZK243]
Western blot: Virus infection Vero cell lysates were stained with mouse anti-ZIKV NS1 monoclonal antibody at 1:2000 dilution. Lane 1: Vero mock cell lysate (4 μg), Lane 2: Vero ZIKV cell lysate (4 μg), Lane 3: Vero DENV1 cell lysate (4 μg), Lane 4: Vero DENV2 cell lysate (4 μg), Lane 5: Vero DENV3 cell lysate (4 μg), Lane 6: Vero DENV 4 cell lysate (4 μg).

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