Leadgene Leadgene
Anti-TERT Antibody [Clone 54-2]
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カタログ番号

LDG0232YA

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  • 概要

    説明

    The TERT antibody targets human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase, which plays a crucial role in maintaining telomere length and cellular immortality. hTERT is highly expressed in most cancer cells, making the hTERT antibody widely used in cancer research and studies related to cellular aging.

    製品ノート

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IFA: 1:50-500

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • 仕様
    • ホスト

      Mouse

      クローナリティ

      Monoclonal

    • アイソタイプ

      IgG1

      クローン名

      Clone 54-2

    • 免疫原

      Human TERT Protein

      反応性

      Human

    • アプリケーション

      ELISA, IFA,WB

      コンジュゲーション

      Unconjugated

    • 濃度

      1 mg/mL

      保存バッファー

      Phosphate Buffered Saline containing 0.03% Proclin300, pH 7.4.

    • 形状

      Liquid

  • 背景
    • 同義語

      Telomerase Reverse Transcriptase (TERT), hTRT, EST2, TP2 (Telomerase-associated Protein 2), Telomerase Catalytic Subunit

  • 使用説明
    • 配送

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      安定性および保存

      This product is stable after storage at:
      ● 2-8°C for 2 weeks under sterile conditions from date of receipt.
      ● -20°C or -80°C for 12 months under sterile conditions from date of receipt.
      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • 画像
    Immunofluorescence: 293T cells were transfected with TERT plasmid and fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with Anti-TERT Antibody [Clone 54-2] (1:50) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with DAPI (Blue).

    Immunofluorescence: 293T cells were transfected with TERT plasmid and fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with Anti-TERT Antibody [Clone 54-2] (1:50) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with DAPI (Blue).

    Western blot: TERT transfected 293T cell lysates were stained with mouse anti-TERT monoclonal antibody.

    Western blot: TERT transfected 293T cell lysates were stained with mouse anti-TERT monoclonal antibody.

    ELISA: Titration curve of anti-TERT antibody in ELISA. Red: Human TERT; Black: BSA (negative control).

    ELISA: Titration curve of anti-TERT antibody in ELISA. Red: Human TERT; Black: BSA (negative control).

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  • 論文

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  • データシート & ドキュメント
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免責事項:For Research Use or Further Manufacturing Only.

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