Anti-ST2L Antibody [Clone 9G15]

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カタログ番号

LDG0046YA

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• 概要

  説明

  The growth stimulation expressed gene 2 (ST2；also known as Interleukin 1 receptor-like 1, IL1RL1), a member of the IL-1 receptor family, has at least three isoforms. ST2L, a main isoform of ST2 proteins, is a transmembrane receptor that is expressed on various inflammatory cells, including T helper 2 (Th2) cells, mast cells, basophils and eosinophils. ST2L is a receptor of Interleukin-33 (IL-33), this ligand-receptor pair plays important role in the regulation of Th2 cell responses and is involved in various inflammatory pathologies.

  製品ノート

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-10000
  IFA: 1:400-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• 仕様

  • ホスト

    Mouse

    クローナリティ

    Monoclonal

  • アイソタイプ

    IgG1

    クローン名

    clone 9G15

  • 免疫原

    ST2L

    反応性

    Human

  • アプリケーション

    ELISA, WB, IFA, FACS

    コンジュゲーション

    Unconjugated

  • 濃度

    1 mg/mL

    保存バッファー

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • 特異性

    ST2L

    形状

    Liquid

• 使用説明

  • 配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    安定性および保存

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• 画像

  Immunofluorescence analysis of Anti-ST2L Antibody [Clone 9G15]
  HT29 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
  Triton X-100. Cells were stained with mouse anti-ST2L monoclonal antibody
  (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
  1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

  FACS analysis of Anti-ST2L Antibody [Clone 9G15]
  HT29 cells were stained with mouse anti-ST2L monoclonal antibody at 1
  μg/ml (red) and without antibody control (black).

  ELISA titration of Anti-ST2L Antibody [Clone 9G15]
  Titration curve of anti-ST2L antibody in ELISA. Red: ST2L; Black: BSA
  (negative control).

  Western blotting analysis of Anti-ST2L Antibody [Clone 9G15]
  HT29 lysate (50 μg) was stained with mouse anti-IL-ST2L monoclonal
  antibody at 1:2000 dilution.

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  合計   (4)

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.