Leadgene Leadgene
Anti-RAGE Antibody [Clone 16-1]
カタログ番号

LDG0039YA

その他のドキュメント
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  • 概要

    説明

    The immunoglobulin superfamily is a group of transmembrane proteins, contains a variable number of disulfide-stabilized motifs similar to immunoglobulins, associated with the adhesion, binding and recognition processes of cells. Receptor for advanced glycation endproducts (RAGE), a member of the immunoglobulin superfamily, not only binds to advanced glycation endproducts but also interacts with multiple ligands having common motifs. These interactions play important roles in various cellular processes, and associated with many human diseases.

    製品ノート

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-10000
    IFA: 1:200-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • 仕様
    • ホスト

      Mouse

      クローナリティ

      Monoclonal

    • アイソタイプ

      IgG1

      クローン名

      clone 16-1

    • 免疫原

      RAGE

      反応性

      Human

    • アプリケーション

      ELISA, WB, IFA, FACS

      コンジュゲーション

      Unconjugated

    • 濃度

      1 mg/mL

      保存バッファー

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • 特異性

      RAGE

      形状

      Liquid

  • 使用説明
    • 配送

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      安定性および保存

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • 画像
    Immunofluorescence analysis of Anti-RAGE Antibody [Clone 16-1] HEK293T cells were transfected with RAGE plasmid and fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-RAGE monoclonal antibody (1:200) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:200, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescence analysis of Anti-RAGE Antibody [Clone 16-1]
    HEK293T cells were transfected with RAGE plasmid and fixed in 4% PFA,
    permeabilized with PBS containing 0.1% Triton X-100. Cells were stained
    with mouse anti-RAGE monoclonal antibody (1:200) followed by secondary
    antibodies (goat anti-Mouse IgG-iFluor 488, 1:200, green) and cell nuclei
    were stained with Hoechst 33342 (Blue).

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  • レビュー

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  • 論文

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  • データシート & ドキュメント
    MSDS
    マニュアル
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免責事項:For Research Use or Further Manufacturing Only.

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