Anti-PGA5 Antibody [Clone 85-5]

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カタログ番号

LDG0054YA

データシート

その他のドキュメント

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• 概要

  説明

  Pepsinogens are members of the peptidases that are secreted by gastric chief cells and are inactive polypeptide proenzymes known as zymogens. They are converted by gastric acid to active pepsins, which are involved in the digestion of proteins. Pepsinogen A5 (PGA5), an isozymogen of pepsinogen A, is the predominant endopeptidase in the gastric acid and is associated with atrophic gastritis and gastritis.

  製品ノート

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IFA: 1:100-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• 仕様

  • ホスト

    Mouse

    クローナリティ

    Monoclonal

  • アイソタイプ

    IgG1

    クローン名

    clone 85-5

  • 免疫原

    The immunogen information used to generate this antibody is proprietary.

    反応性

    Human

  • アプリケーション

    ELISA, WB, IFA, FACS

    コンジュゲーション

    Unconjugated

  • 濃度

    1 mg/mL

    保存バッファー

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • 特異性

    PGA5

    形状

    Liquid

• 使用説明

  • 配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    安定性および保存

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• 画像

  Immunofluorescence analysis of Anti-PGA5 Antibody [Clone 85-5]
  HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
  Triton X-100. Cells were stained with mouse anti-PGA5 monoclonal antibody
  (1:100) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
  1:200, green) and cell nuclei were stained with Hoechst 33342 (Blue).

  FACS analysis of Anti-PGA5 Antibody [Clone 85-5]
  K562 cells were stained with mouse anti-PGA5 monoclonal antibody at 2
  μg/ml (red) and without antibody control (black).

  ELISA analysis of Anti-PGA5 Antibody [Clone 85-5]
  The specificity of anti-PGA5 antibody recognize recombinant proteins in
  ELISA. Red: Porcine pepsin; Orange: PGA5; Green: Pepsinogen I; Blue:
  Pepsinogen II; Black: BSA (negative control).

  Western blotting analysis of Anti-PGA5 Antibody [Clone 85-5]
  Recombinant proteins and mouse stomach lysates were stained with mouse
  anti-PGA5 monoclonal antibody at 1:5000 dilution. Lane 1: Purified human
  pepsinogen I (25 ng). Lane 2: Purified human pepsinogen II (25 ng). Lane 3:
  Mouse stomach lysate (30 μg). Lane 4: Human gastric juice (10 μL).

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  合計   (4)

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.