カタログ番号

LDG0042YA

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概要

説明

Matrix metalloproteinases (MMPs) is a group of proteolytic enzymes that targets many extracellular proteins including other proteases, growth factors, cell surface receptors and adhesion molecules, plays an important role in many physiological and pathological processes. Matrix metalloproteinase-7 (MMP-7；matrilysin-1；PUMP-1), a member of the MMP family, can degrade a large series of proteins of the extracellular matrix and other substrates. MMP-7 is an important factor for normal tissue remodeling and wound healing and is associated with the occurrence and development of various tumors, thus may be a tumor biomarker and therapeutic target.

製品ノート

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-10000
IFA: 1:200-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
仕様
- ホスト
  
  Mouse
  
  クローナリティ
  
  Monoclonal
- アイソタイプ
  
  IgG2a
  
  クローン名
  
  clone 1-4
- 免疫原
  
  MMP7
  
  反応性
  
  Human
- アプリケーション
  
  ELISA, WB, IFA, FACS
  
  コンジュゲーション
  
  Unconjugated
- 濃度
  
  1 mg/mL
  
  保存バッファー
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- 特異性
  
  Pro-MMP7 & mature-MMP7
  
  形状
  
  Liquid
使用説明
- 配送
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  安定性および保存
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
画像

Immunofluorescence analysis of Anti-MMP7 Antibody [Clone 1-4]
A549 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
Triton X-100. Cells were stained with mouse anti-MMP7 monoclonal antibody
(1:200) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

FACS analysis of Anti-MMP7 Antibody [Clone 1-4]
A549 cells were stained with mouse anti-MMP7 monoclonal antibody at 2
μg/ml (red) and without antibody control (black).

ELISA titration of Anti-MMP7 Antibody [Clone 1-4]
Titration curve of anti-MMP7 antibody in ELISA. Red: MMP7 (active); Green:
MMP7 (proenzyme); Black: BSA (negative control).

Western blotting analysis of Anti-MMP7 Antibody [Clone 1-4]
Recombinant proteins and LPS treat MMP7 transfected 293T cell lysates
were stained with mouse anti-MMP7 (1-4) monoclonal antibody at 1:5000
dilution. Lane 1: recombinant MMP7 (proenzyme) protein (50 ng). Lane 2:
recombinant MMP7 (active) protein (50 ng). Lane 3: 293T cell lysates (30
μg). Lane 4: lysates from LPS treat 293T cells (30 μg). Lane 5: MMP7
transfected 293T cell lysates (30 μg). Lane 6: lysates from LPS treat MMP7
transfected 293T cell lysates (30 μg). The expression of Actin was as the
internal control.

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