Anti-IL-8 Antibody [Clone 58-1]

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カタログ番号

LDG0030YA

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• 概要

  説明

  The CXC chemokine is a group of secreted proteins, attracts neutrophils and lymphocytes. Interleukin-8 (IL-8, or CXCL8), a member of the CXC chemokines, is a proinflammatory cytokine produced by macrophages and other cell types like endothelial cells, epithelial cells and airways smooth muscle cells. This cytokine mainly attracts and activates neutrophils to the site of inflammation and also plays an important role in cell proliferation, tissue remodeling and angiogenesis.

  製品ノート

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IFA: 1:500-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• 仕様

  • ホスト

    Mouse

    クローナリティ

    Monoclonal

  • アイソタイプ

    IgG1

    クローン名

    clone 58-1

  • 免疫原

    Interleukin 8

    反応性

    Human

  • アプリケーション

    ELISA, WB, IFA, FACS

    コンジュゲーション

    Unconjugated

  • 濃度

    1 mg/mL

    保存バッファー

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • 特異性

    Interleukin 8

    形状

    Liquid

• 使用説明

  • 配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    安定性および保存

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• 画像

  Immunofluorescence analysis of Anti-IL-8 Antibody [Clone 58-1]
  HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
  Triton X-100. Cells were stained with mouse anti-IL-8 monoclonal antibody
  (1:200) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
  1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

  FACS analysis of Anti-IL-8 Antibody [Clone 58-1]
  Human peripheral blood mononuclear cells (PBMC) were stained with mouse
  anti-IL-8 monoclonal antibody at 2 μg/ml (red) and without antibody control
  (black).

  ELISA titration of Anti-IL-8 Antibody [Clone 58-1]
  Titration curve of anti-IL-8 antibody in ELISA. Red: IL-8; Black: BSA (negative
  control).

  Western blotting analysis of Anti-IL-8 Antibody [Clone 58-1]
  Recombinant IL-8 protein (100 ng) was stained with mouse anti-IL-8
  monoclonal antibody at 1:5000 dilution.

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.