Anti-His Tag Antibody [Clone 14-4]

カタログ番号

LDG0001YB

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• 概要

  説明

  Mouse anti-His tag mAb is specific to 6X His tag, which can be applied to several immunoassays, including ELISA, WB, IP, IFA, and FACS. This antibody has been identified in several sources (bacteria, yeast, and mammalian cells) of His tag fusion protein.

  製品ノート

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IP: 1:500-2000
  IFA: 1:500-1000
  FACS: 1:500-1000
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• 仕様

  • ホスト

    Mouse

    クローナリティ

    Monoclonal

  • アイソタイプ

    IgG1

    クローン名

    clone 14-4

  • 免疫原

    6X His tag fusion protein

    アプリケーション

    ELISA, WB, IP, IFA, FACS

  • コンジュゲーション

    Unconjugated

    濃度

    1 mg/mL

  • 保存バッファー

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    特異性

    Recognizes 6X His tag on either the N- or C-terminus of recombinant fusion protein.

  • 形状

    Liquid

• 使用説明

  • 配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    安定性および保存

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• 画像

  Western blotting analysis of anti-His mAb (1:5000)
  Lane 1: 293T whole cell lysate-untransfected
  Lane 2: 293T whole cell lysate-transfected
  Lysate/proteins at 20 ug per lane

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  合計   (1)

• レビュー

  

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• 論文

  1. Cheng YL et al. Frontiers in immunology. 2022; 13:941923.
  2. Yang YH et al. Viruses. 2022; 14(5):925.
  3. Lai YC et al. Frontiers in immunology. 2022; 13:868724.

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.