Anti-HDGF Antibody [Clone 83-5]

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カタログ番号

LDG0040YA

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その他のドキュメント

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• 概要

  説明

  Hepatoma-derived growth factor (HDGF；also known as high mobility group protein 1-like 2, HMG-1L2) is a heparin-binding protein that is ubiquitously expressed in normal tissues and abnormally expressed in various malignancies. HDGF has various intracellular functions and localizes to the nucleus, and thus it is characterized as a mitogen for many cell types. As its promoting growth, suppressing differentiation and exhibiting angiogenic properties, HDGF plays a critical role in tumor progression.

  製品ノート

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-10000
  IFA: 1:200-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• 仕様

  • ホスト

    Mouse

    クローナリティ

    Monoclonal

  • アイソタイプ

    IgG1

    クローン名

    clone 83-5

  • 免疫原

    HDGF

    反応性

    Human

  • アプリケーション

    ELISA, WB, IFA, FACS

    コンジュゲーション

    Unconjugated

  • 濃度

    1 mg/mL

    保存バッファー

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • 特異性

    HDGF

    形状

    Liquid

• 使用説明

  • 配送

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    安定性および保存

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• 画像

  Immunofluorescence analysis of Anti-HDGF Antibody [Clone 83-5]
  Cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton
  X-100. Cells were stained with anti-HDGF monoclonal antibody (1:200)
  followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:200,
  green) and cell nuclei were stained with Hoechst 33342 (Blue).

  Western blotting analysis of Anti-HDGF Antibody [Clone 83-5]
  Tissue lysate was stained with anti-HDGF monoclonal antibody at 1:5000
  dilution. Lane 1: Mouse liver lysate (50 μg). Lane 2: Rat liver lysate (50 μg).

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  合計   (2)

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• 論文

  

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• データシート & ドキュメント

  データシート

  MSDS

  マニュアル

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免責事項：For Research Use or Further Manufacturing Only.