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Leadgene Leadgene
Anti-EGFP Antibody [Clone 42-2]
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LDG0008YB

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  • 概要

    説明

    EGFP is a 27 kDa fluorescent protein derived from Aequorea victoria.EGFP has F64L and S65T mutations which result in brighter green fluorescence.

    製品ノート

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IP: 1:500-2000
    IFA: 1:500-1000
    FACS: 1:500-1000

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • 仕様

    • ホスト

      Mouse

      クローナリティ

      Monoclonal

    • アイソタイプ

      IgG2a

      クローン名

      clone 42-2

    • 免疫原

      Recombinant EGFP protein

      アプリケーション

      ELISA, WB, IP, IFA, FACS

    • コンジュゲーション

      Unconjugated

      濃度

      1 mg/mL

    • 保存バッファー

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

      特異性

      Recognizes EGFP

    • 形状

      Liquid

  • 使用説明

    • 配送

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      安定性および保存

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • 画像
    Immunofluorescent: 293T cells were transfected with an EGFP fusion protein and fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were either natural fluorescence (green) or stained with mouse anti-EGFP monoclonal antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 594, 1:400, red) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescent: 293T cells were transfected with an EGFP fusion protein and fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were either natural fluorescence (green) or stained with mouse anti-EGFP monoclonal antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 594, 1:400, red) and cell nuclei were stained with Hoechst 33342 (Blue).

    Western blotting analysis of anti-EGFP mAb (1:2000) Lane 1: 293T cell lysate Lane 2: EGFP cell lysate Lane 3: mcherry cell lysate Lysate at 6 μg per lane

    Western blotting analysis of anti-EGFP mAb (1:2000)
    Lane 1: 293T cell lysate
    Lane 2: EGFP cell lysate
    Lane 3: mcherry cell lysate
    Lysate at 6 μg per lane

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  • データシート & ドキュメント
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免責事項：For Research Use or Further Manufacturing Only.

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