カタログ番号

LDG0013YA

データシート

その他のドキュメント

パッケージと価格を読み込み中...

COA 検索

概要

説明

Dengue virus belongs to the family Flaviviridae, genus Flavivirus and consists of four distinct serotypes (DENV1 to 4). Dengue virus is transmitted by mosquitos majorly of species Aedes aegypti and Aedes Albopictus that widespread around tropics and subtropics. During dengue virus maturation, host protease furin cleaves prM to M and pr. The peripheral pr peptide is retained on the virus particle at acidic pH. This virus-pr complex does not bind target membranes and thus to inhibit virus-membrane interaction and blocks virus fusion.

製品ノート

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:500-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
仕様
- ホスト
  
  Mouse
  
  クローナリティ
  
  Monoclonal
- アイソタイプ
  
  IgG1
  
  クローン名
  
  clone 47-2
- 免疫原
  
  Dengue virus Pr
  
  反応性
  
  Dengue virus
- アプリケーション
  
  ELISA, WB, IFA, FACS
  
  コンジュゲーション
  
  Unconjugated
- 濃度
  
  1 mg/mL
  
  保存バッファー
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- 特異性
  
  pr
  
  形状
  
  Liquid
使用説明
- 配送
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  安定性および保存
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
画像

Immunofluorescence analysis of Anti-DENV pr Antibody [Clone 47-2]
Control BHK cells (uninfected) and Virus (DENV 2) infection BHK cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100.
Cells were stained with mouse anti-DENV pr monoclonal antibody followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

ELISA titration of Anti-DENV pr Antibody [Clone 47-2]
Titration curve of anti-DENV pr antibody in ELISA. Red: pr; Black: BSA (negative control).

Western blotting analysis of Anti-DENV pr Antibody [Clone 47-2]
Virus infection BHK cell lysates were stained with mouse anti-DENV pr monoclonal antibody at 1:1000 dilution.
Lane 1: BHK cell lysate (10 μg), Lane 2: ZIKV infection (10 μg), Lane 3: DENV1 infection (10 μg), Lane 4: DENV2 infection (10 μg), Lane 5: DENV3 infection (10 μg), Lane 6: DENV4 infection (10 μg).

1

/

3

合計 (3)
レビュー

この製品についてのご経験を共有してください。下のリンクから投稿いただけます。

レビューを投稿
論文

現在論文はありません。下のリンクからお知らせください。

論文を投稿
データシート & ドキュメント

データシート

MSDS

マニュアル

1

/

1