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LDG0007YA

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概要

説明

Dengue virus belongs to the family Flaviviridae, genus Flavivirus and consists of four distinct serotypes (DENV1 to 4). Dengue virus is transmitted by mosquitos majorly of species Aedes aegypti and Aedes Albopictus that widespread around tropics and subtropics. NS5 is the largest and the most conserved protein of Dengue virus, acting as both a RNA-dependent RNA polymerase and a RNA methyltransferase enzyme. The NS5 proteins are important for viral replication and can down-regulate the host immune interferon response.

製品ノート

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:500-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
仕様
- ホスト
  
  Mouse
  
  クローナリティ
  
  Monoclonal
- アイソタイプ
  
  IgG2b
  
  クローン名
  
  clone 37-2
- 免疫原
  
  Dengue virus NS5
  
  反応性
  
  Dengue virus
- アプリケーション
  
  ELISA, WB, IFA, FACS
  
  コンジュゲーション
  
  Unconjugated
- 濃度
  
  1 mg/mL
  
  保存バッファー
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- 特異性
  
  NS5
  
  形状
  
  Liquid
使用説明
- 配送
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  安定性および保存
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
画像

Immunofluorescence analysis of Anti-DENV NS5 Antibody [Clone 37-2]
Control Vero cells (uninfected) and Virus (DENV 1,2,4) infection Vero cells
were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-
100. Cells were stained with mouse anti-DENV NS5 monoclonal antibody
followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400,
green) and cell nuclei were stained with Hoechst 33342 (Blue).

ELISA titration of Anti-DENV NS5 Antibody [Clone 37-2]
Titration curve of anti-DENV NS5 antibody in ELISA. Red: NS5; Black: BSA
(negative control).

Western blotting analysis of Anti-DENV NS5 Antibody [Clone 37-2]
Virus infection Vero cell lysates were stained with mouse anti-DENV NS5
monoclonal antibody at 1:1000 dilution. Lane 1: Vero cell lysate (10 μg), Lane
2: ZIKV infection (10 μg), Lane 3: DENV1 infection (10 μg), Lane 4: DENV2
infection (10 μg), Lane 5: DENV3 infection (10 μg), Lane 6: DENV4 infection
(10 μg).

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