Vaccinia Capping Enzyme

Katalognummer

LDG0013RI

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• Übersicht

  Beschreibung

  This capping enzyme contains three enzymatic activities (RNA triphosphatase, guanylyltransferase and guanine methyltransferase) to forming a Cap 0 structure (m7Gppp5'N). In the presence of the vaccine capping enzyme, GTP and SAM (methyl donor) the In Vitro Transcripts mRNA can be efficient add capped in less one hour.

  Produktnotiz

  Reactionshould be performed under RNase free condition. Use nuclease-free tubes, reagents and water to avoid RNase contamination. Also, wear gloves when working with RNA.

  Komponenten

  Package	Items	Quantity
  500 U	Vaccinia Capping Enzyme	1 vial (500 U)
  	10X Capping Buffer	1 vial (100 μL)

  • 500 U

    Items

    Vaccinia Capping Enzyme

    Quantity

    1 vial (500 U)

    Items

    10X Capping Buffer

    Quantity

    1 vial (100 μL)

• Spezifikationen

  • Expressionssystem

    Escherichia coli

    Anwendung

    In vitro transcription, RNA amplification, miRNA and siRNA synthesis

  • Konzentration

    10 U/µL

    Lagerpuffer

    Vaccinia capping enzyme is supplied in 50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton® X-100 and 50% Glycerol.

  • Reinheit

    >95% as determined by SDS-PAGE analysis.

    Einheitsdefinition

    One unit of Vaccinia Capping Enzyme is defined as the amount of enzyme required to incorporate 10 pmol of (α32P) GTP into an 80 nt transcript in 1 hour at 37°C

  • Form

    Liquid

• Anleitung

  • Versand

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stabilität & Lagerung

    This product is stable after storage at:

    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.

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• Datenblatt & Dokumente

  Datenblatt

  MSDS

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Haftungsausschluss：For Research Use or Further Manufacturing Only.