Beschreibung
Ribonuclease R (RNase R), derived from E. coli, is a magnesium-dependent exoribonuclease operating in a 3'→5' direction. Its primary function is to degrade linear RNAs while sparing lariat or circular RNA structures. Most cellular RNAs undergo complete digestion by RNase R, with exceptions such as tRNAs, 5S RNA, and intron lariats.
RNase R effectively breaks down linear and Y-structured RNAs but does not affect lariats or circular RNAs. This property makes it useful for selectively enriching circular RNAs, which can be employed in protein production or intronic cDNA library construction.
Produktnotiz
- RNase R activity is optimal in the presence of low magnesium concentrations ranging from 0.1 to 1.0 mM. However, the effectiveness of RNase R can be hindered by low EDTA levels in RNA substrate solutions. To mitigate this negative impact, additional MgCl₂ can be added, up to a final concentration of 1 mM, to counteract the presence of EDTA in the substrate.
- The enzyme exhibits its best performance at a temperature of 37°C.
Komponenten
500 U
Items
RNase R
Quantity
1 vial (500 U)
Items
10X Reaction buffer
Quantity
1 vial (500 μL)
