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LGX-23001 Endoglycosidase H (Endo H) [GMP-Grade]
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LGX-23001

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  • Übersicht

    Beschreibung

    Endoglycosidase H (Endo H) is a highly specific recombinant glycosidase that cleaves within the chitobiose core of high-mannose and some hybrid N-linked oligosaccharides from glycoproteins. It hydrolyzes the β-1,4 linkage between the two N-acetylglucosamine (GlcNAc) residues, leaving a single GlcNAc attached to the asparagine residue of the peptide chain. However, Endo H does not cleave complex-type N-glycans.
    Protein glycosylation is a crucial post-translational modification that influences many biological functions, such as protein folding, quality control, stability, intracellular transportation, cellular and immune responses. During eukaryotic protein expression, heterogeneous glycosylation patterns can complicate purification and downstream applications.
    By using Leadgene® Endo H, high-mannose and hybrid N-glycans can be efficiently removed, improving protein homogeneity, facilitating purification, and enhancing protein purification efficiency, which indirectly increases the recoverable yield of proteins and antibodies.

    Produktnotiz

    • LGX-23001 Endoglycosidase H (Endo H) has been filed with the FDA Drug Master Files (DMF).

    Komponenten

    Package

    Items

    Quantity

    100 μL-50 KU (Endo H)

    LGX-23001 Endo H

    1 vial (100 µL/vial, 500 U/µL)

    100 μL-50 KU (Endo H & Buffer Set)

    LGX-23001 Endo H

    1 vial (100 µL/vial, 500 U/µL)

    LGX-23002 Endo H Buffer Set (10X glycoprotein denaturing buffer)

    1 vial (1 mL/vial)

    LGX-23002 Endo H Buffer Set (10X reaction buffer)

    1 vial (1 mL/vial)

    1 mL-500 KU (Endo H)

    LGX-23001 Endo H

    1 vial (1 mL/vial, 500 U/µL)

    1 mL-500 KU (Endo H & Buffer Set)

    LGX-23001 Endo H

    1 vial (1 mL/vial, 500 U/µL)

    LGX-23002 Endo H Buffer Set (10X glycoprotein denaturing buffer)

    1 vial (1 mL/vial)

    LGX-23002 Endo H Buffer Set (10X reaction buffer)

    1 vial (1 mL/vial)

    • 100 μL-50 KU (Endo H)

      Items

      LGX-23001 Endo H

      Quantity

      1 vial (100 µL/vial, 500 U/µL)

    • 100 μL-50 KU (Endo H & Buffer Set)

      Items

      LGX-23001 Endo H

      Quantity

      1 vial (100 µL/vial, 500 U/µL)

      Items

      LGX-23002 Endo H Buffer Set (10X glycoprotein denaturing buffer)

      Quantity

      1 vial (1 mL/vial)

      Items

      LGX-23002 Endo H Buffer Set (10X reaction buffer)

      Quantity

      1 vial (1 mL/vial)

    • 1 mL-500 KU (Endo H)

      Items

      LGX-23001 Endo H

      Quantity

      1 vial (1 mL/vial, 500 U/µL)

    • 1 mL-500 KU (Endo H & Buffer Set)

      Items

      LGX-23001 Endo H

      Quantity

      1 vial (1 mL/vial, 500 U/µL)

      Items

      LGX-23002 Endo H Buffer Set (10X glycoprotein denaturing buffer)

      Quantity

      1 vial (1 mL/vial)

      Items

      LGX-23002 Endo H Buffer Set (10X reaction buffer)

      Quantity

      1 vial (1 mL/vial)

  • Spezifikationen

    • Konzentration

      500 U/μL

      Reinheit

      >95% as determined by SDS-PAGE analysis.

    • Aktivität

      One unit of Endoglycosidase H cleaves > 95% of the carbohydrate from 10 μg of denatured RNase B in a total reaction volume of 10 μL at 37°C for 1 h.

      Endotoxingehalt

      <0.05 EU per 1 µg of the protein by the LAL method.

    • Mykoplasmen

      Negative

      Form

      Liquid

    • Standard

      Microorganism Test: Negative

  • Anleitung

    • Versand

      The product is shipped at either 2-8°C or frozen. Upon receipt, store it immediately at 2-8°C or lower for long-term storage.

      Stabilität & Lagerung

      • When unopened and stored at 2-8ºC, the reagent can be stored for at least 12 months. The expiration date is indicated on the outer packaging.
      • Once opened, the product should be used within two weeks. Any remaining reagent should be refrigerated in 2-8℃ immediately after each use.
      • The enzyme should be diluted and prepared on the day of use.

    • Herstellungsspezifikationen

      Leadgene GMP-grade proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

      • Animal-free reagent and laboratory
      • Manufactured and tested under GMP guideline
      • Testing and traceability of raw material
      • Records of the maintenance and equipment calibration
      • Personnel training records
      • Batch-to-batch consistency
      • Documentation of QA control and process changes
      • Manufactured and tested under an ISO 13485:2016 certified quality management system
      • Stability monitor of product shelf-life

      Standard

      1. After thawing, the buffers may crystallize, which is a normal occurrence. Warm crystallized buffer until the salt crystals return to solution. Ensure that your components return to RT before use in the assay.
      2. Please fine-tune the input sample volume to find the optimal condition for your assay.
      3. Once optimize for the cleavage condition, the cleavage reactions can be scaled up to cleave a large amount of the target fusion protein.

  • Bild
    Endo H cleaves high mannose and hybrid-type glycans.

    Endo H cleaves high mannose and hybrid-type glycans.

    The standard assay was performed by incubating 1 unit of Endo H and 10 µg of RNase B under the above conditions. SDS-PAGE analysis of RNase B digested with Endoglycosidase H.

    The standard assay was performed by incubating 1 unit of Endo H and 10 µg of RNase B under the above conditions. SDS-PAGE analysis of RNase B digested with Endoglycosidase H.

    SDS-PAGE analysis of recombinant Endoglycosidase H

    SDS-PAGE analysis of recombinant Endoglycosidase H

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    Datenblatt
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