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Übersicht

Beschreibung

PNGase F is an enzyme used in biochemistry and molecular biology to remove N-linked glycans from glycoproteins. By using PNGase F, researchers can enzymatically cleave between these sugar chains and asparagine residues of glycoproteins, allowing for the study of protein structure and function, particularly in glycosylation research.

Komponenten

Package	Items	Quantity
15 KU	LeadGMP® PNGase F	1 vial (400 U/μL)
	10X Glycoprotein Denaturing Buffer	1 vial (1 mL)
	10X Reaction buffer	1 vial (1 mL)
	10% NP-40	1 vial (1 mL)
75 KU	LeadGMP® PNGase F	1 vial (400 U/μL)
	10X Glycoprotein Denaturing Buffer	1 vial (1 mL)
	10X Reaction buffer	1 vial (1 mL)
	10% NP-40	1 vial (1 mL)

15 KU
Items
LeadGMP® PNGase F
Quantity
1 vial (400 U/μL)
Items
10X Glycoprotein Denaturing Buffer
Quantity
1 vial (1 mL)
Items
10X Reaction buffer
Quantity
1 vial (1 mL)
Items
10% NP-40
Quantity
1 vial (1 mL)
75 KU
Items
LeadGMP® PNGase F
Quantity
1 vial (400 U/μL)
Items
10X Glycoprotein Denaturing Buffer
Quantity
1 vial (1 mL)
Items
10X Reaction buffer
Quantity
1 vial (1 mL)
Items
10% NP-40
Quantity
1 vial (1 mL)

Spezifikationen
- Expressionssystem
  
  Escherichia coli
  
  Konzentration
  
  400 U /μL
- Lagerpuffer
  
  20mM Tris-HCl, 50mM NaCl, 5mM EDTA, pH 7.5
  
  Reinheit
  
  >95% as determined by SDS-PAGE analysis.
- Einheitsdefinition
  
  One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 20 µL.
  
  Endotoxingehalt
  
  <0.05 EU per 1 µg of the protein by the LAL method.
- Mykoplasmen
  
  Not detected
  
  Form
  
  Liquid
Anleitung
- Stabilität & Lagerung
  
  This product is stable after storage at:
  - -20°C for long-term storage under sterile conditions. Avoid repeated free-thaw cycles.
  Herstellungsspezifikationen
  
  LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:
  - Animal-free reagent and laboratory
  - Manufactured and tested under GMP guideline
  - Testing and traceability of raw material
  - Records of the maintenance and equipment calibration
  - Personnel training records
  - Batch-to-batch consistency
  - Documentation of QA control and process changes
  - Manufactured and tested under an ISO 13485:2016 certified quality management system
  - Stability monitor of product shelf-life
Bild

The intracellular PD-L1 protein was digested with LeadGMP® PNGase F and analyzed by Western blotting. The assay was performed by incubating 400 U PNGase F and 20 µg of glycoprotein from whole cell lysate.

SDS-PAGE analysis of recombinant PNGase F

PNGase F cleaves high mannose, hybrid-type and complex structure glycans.

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