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Ursprungsart
Human
Expressionssystem
Escherichia coli
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Affinitäts-Tag
His Tag (C-term)
Lagerpuffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
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Reinheit
>97% as determined by SDS-PAGE analysis.
Molekulargewicht
The protein has a calculated MW of 18.3 kDa.
The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis). -
Aktivität
Measured by its ability to induce PHA-activated human PBMCs proliferation. The ED₅₀ for this effect is 0.5-4 ng/mL. The specific activity of recombinant human IL-7 is > 1 x 10⁸ unit/mg, which is calibrated against the human IL-7 WHO Reference Reagent (NIBSC code: 90/530).
Endotoxingehalt
<0.05 EU per 1 µg of the protein by the LAL method.
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Mykoplasmen
Not detected
Form
Lyophilized
Mehr Bilder
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Spezifikationen
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Hintergrund
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Hintergrund
Interleukin-7 (IL-7) is a multipotent cytokine belonged to one of the members of IL-2 superfamily. IL-7 has diverse effects on the hematopoietic and immune regulations. IL-7 is a trophic factor that is necessary for both B cell and T cell proliferation and development. In addition, it presents potential antitumor effects in tumors such as glioma, melanoma, lymphoma, leukemia, prostate cancer, and glioblastoma.
Synonyme
Interleukin-7
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Uniprot ID
P13232
Sequenznotiz
Asp26-His177
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Anleitung
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Rekonstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Versand
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stabilität & Lagerung
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Herstellungsspezifikationen
LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:
- Animal-free reagent and laboratory
- Manufactured and tested under GMP guideline
- Testing and traceability of raw material
- Records of the maintenance and equipment calibration
- Personnel training records
- Batch-to-batch consistency
- Documentation of QA control and process changes
- Manufactured and tested under an ISO 13485:2016 certified quality management system
- Stability monitor of product shelf-life
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Bild
![Activity analysis of recombinant LeadGMP® Human IL-7 Protein, His Tag, E. coli. Measured by its ability to induce PHA-activated human PBMCs proliferation. The ED₅₀ for this effect is 0.5-4 ng/mL. The specific activity of recombinant human IL-7 is > 1 x 10⁸ unit/mg, which is calibrated against the human IL-7 WHO Reference Reagent (NIBSC code: 90/530).]()
Activity analysis of recombinant LeadGMP® Human IL-7 Protein, His Tag, E. coli.
Measured by its ability to induce PHA-activated human PBMCs proliferation. The ED₅₀ for this effect is 0.5-4 ng/mL. The specific activity of recombinant human IL-7 is > 1 x 10⁸ unit/mg, which is calibrated against the human IL-7 WHO Reference Reagent (NIBSC code: 90/530).![SDS-PAGE analysis of LeadGMP® IL-7, Human]()
SDS-PAGE analysis of LeadGMP® IL-7, Human
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Bewertung
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Publikation
Derzeit gibt es keine Publikationen. Nutzen Sie den Link unten, um uns zu informieren.
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Datenblatt & Dokumente
1/1
Haftungsausschluss：For Research Use or Further Manufacturing Only.
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