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Ursprungsart
Human
Expressionssystem
Escherichia coli
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Affinitäts-Tag
His Tag (C-term)
Lagerpuffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
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Reinheit
>97% as determined by SDS-PAGE analysis.
Molekulargewicht
The protein has a calculated MW of 16.2 kDa.
The protein migrates as 15-19 kDa under reducing condition (SDS-PAGE analysis). -
Aktivität
Measure by its ability to enhance IFN gamma secretion in NK-92 cells. The ED₅₀ for this effect is <10 ng/mL.
Endotoxingehalt
<0.05 EU per 1 µg of the protein by the LAL method.
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Mykoplasmen
Not detected
Form
Lyophilized
Mehr Bilder
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Spezifikationen
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Hintergrund
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Hintergrund
Interleukin-21 (IL-21) belongs to the IL-15/IL-21 family, which exerts pleiotropic immune regulations. IL-21 produced primarily by natural killer T (NKT) cells, T follicular helper (TFH) cells and TH17 cells. As a pleiotropic cytokine, IL-21 has been shown to regulate both innate and humoral immunity. It has potent inhibitory activity towards the activation and maturation of granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced dendritic cells (DCs). In B cells, IL-21 has a major role in the development of immunoglobulin responses. In T cells, it is required to facilitate the functional differentiation of several CD4+ T cell subsets. In addition, the ability of IL-21 to enhance the cytotoxic activity of both CD8+ T cells and NK cells makes it as a potential antitumor agent.
Synonyme
Interleukin-21, Za11
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Uniprot ID
Q9HBE4
Sequenznotiz
Gln32-Ser162
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Anleitung
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Rekonstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Versand
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stabilität & Lagerung
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Herstellungsspezifikationen
LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:
- Animal-free reagent and laboratory
- Manufactured and tested under GMP guideline
- Testing and traceability of raw material
- Records of the maintenance and equipment calibration
- Personnel training records
- Batch-to-batch consistency
- Documentation of QA control and process changes
- Manufactured and tested under an ISO 13485:2016 certified quality management system
- Stability monitor of product shelf-life
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Bild
![The purity of LeadGMP® Human IL-21 Protein is greater than 95% as determined by RP-HPLC.]()
The purity of LeadGMP® Human IL-21 Protein is greater than 95% as determined by RP-HPLC.
![LeadGMP® Human IL-21 Protein, His Tag, E. coli (LDG011PHE-GMP) enhances IFN gamma secretion in NK-92 cells, with the ED50 <10 ng/mL]()
LeadGMP® Human IL-21 Protein, His Tag, E. coli (LDG011PHE-GMP) enhances IFN gamma secretion in NK-92 cells, with the ED50 <10 ng/mL
![SDS-PAGE analysis of LeadGMP® IL-21, Human]()
SDS-PAGE analysis of LeadGMP® IL-21, Human
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Bewertung
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Publikation
Derzeit gibt es keine Publikationen. Nutzen Sie den Link unten, um uns zu informieren.
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Datenblatt & Dokumente
1/1
Haftungsausschluss：For Research Use or Further Manufacturing Only.
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