-
Ursprungsart
Human
Expressionssystem
Escherichia coli
-
Affinitäts-Tag
His Tag (N-term)
Lagerpuffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
-
Reinheit
>98% as determined by SDS-PAGE analysis.
Molekulargewicht
The protein has a calculated MW of 15.4 kDa.
The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis). -
Aktivität
Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <80 pg/mL. The specific activity of recombinant human GM-CSF is approximately >1 x 10⁷ IU/mg.
Endotoxingehalt
<0.05 EU per 1 µg of the protein by the LAL method.
-
Mykoplasmen
Not detected
Form
Lyophilized
-
Spezifikationen
-
Hintergrund
-
Hintergrund
Granulocyte-macrophage colony-stimulating factor (GM-CSF) was first identified as a growth factor due to its ability to induce proliferation and differentiation of bone marrow progenitors into granulocytes and macrophages. GM-CSF is produced by multiple cell types including activated T cells, B cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. GM-CSF stimulates stem cells to produce granulocytes and monocytes functions as a cytokine.
Synonyme
Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor , CSF, Molgramostin, Sargramostim
-
Uniprot ID
P04141
Sequenznotiz
Ala18-Glu144
-
-
Anleitung
-
Rekonstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Versand
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
-
Stabilität & Lagerung
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Herstellungsspezifikationen
LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:
- Animal-free reagent and laboratory
- Manufactured and tested under GMP guideline
- Testing and traceability of raw material
- Records of the maintenance and equipment calibration
- Personnel training records
- Batch-to-batch consistency
- Documentation of QA control and process changes
- Manufactured and tested under an ISO 13485:2016 certified quality management system
- Stability monitor of product shelf-life
-
-
Bild
![SDS-PAGE analysis of LeadGMP® GM-CSF, Human]()
SDS-PAGE analysis of LeadGMP® GM-CSF, Human
1/1 -
Bewertung
Helfen Sie anderen, mehr über dieses Produkt zu erfahren. Nutzen Sie den Link unten, um Ihre Erfahrung zu teilen.
-
Publikation
Derzeit gibt es keine Publikationen. Nutzen Sie den Link unten, um uns zu informieren.
-
Datenblatt & Dokumente
1/1
Haftungsausschluss：For Research Use or Further Manufacturing Only.
