Beschreibung
Glycerol dehydrogenase is an enzyme that catalyzes the oxidation of glycerol to dihydroxyacetone, using NAD+ as a cofactor, which is reduced to NADH in the process. This enzyme plays a key role in the metabolism of glycerol and is involved in pathways such as gluconeogenesis and lipid metabolism. Glycerol dehydrogenase is utilized in various industrial applications, including the production of dihydroxyacetone for cosmetic and pharmaceutical products.
Expressionssystem
Escherichia coli
Aktivität
≥40 U/mg
Einheitsdefinition
One unit causes the formation of one micromole of NADH per minute under the following conditions：0.1 M Carbonate buffer pH 11, 0.1 M Glycerol, 1 mM NAD+ and 33 mM Ammonium sulfate.
Form
Lyophilized (Light brown amorphous powder)
Rekonstitution
It is recommended to weigh and reconstitute 30 mg of lyophilized powder in 157 μL double-distilled water directly and incubate the solution for at least 20 mins to ensure sufficient re-dissolved.
Versand
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stabilität & Lagerung
This product is stable at -20°C for long-term storage under sterile conditions.
Avoid repeated free-thaw cycles.
The effects of various chemicals on Glycerol dehydrogenase.
The enzyme was incubated with the chemicals at 25°C for 1 hour.
pH stability of Glycerol dehydrogenase.
The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 20 hours at 25°C. pH 6.0, 0.1 M Sodium citrate buffer; pH 7.5-8.0, 0.1 M Potassium phosphate buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.
Thermal stability of Glycerol dehydrogenase.
The enzyme powder was reconstituted by double-distilled water and treated with different temperature for 15 minutes. Final concentration: 16 U/ mL
pH activity of Glycerol dehydrogenase.
The buffer conditions with various pH values were used in the reaction at 25°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.5-8.0, 0.1 M Potassium phosphate buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.
Temperature activity of Glycerol dehydrogenase.
The enzyme reactions in 0.1 M Carbonate-bicarbonate buffer, pH 11.0, were carried out under different temperature.
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Haftungsausschluss：For Research Use or Further Manufacturing Only.
