eSpCas9(1.1)

Katalognummer

LDG002POE

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• Spezifikationen

  • Ursprungsart

    Streptococcus pyogenes

    Expressionssystem

    Escherichia coli

  • Affinitäts-Tag

    His-MBP Tag (N-term)

    Lagerpuffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Reinheit

    >98% as determined by SDS-PAGE analysis.

    Molekulargewicht

    The protein has a calculated MW of 201 kDa.
    The protein migrates about 180 kDa under reducing condition (SDS-PAGE analysis).

  • Endotoxingehalt

    <0.1 EU per 1 μg of the protein by the LAL method.

    Form

    Lyophilized

• Hintergrund

  • Hintergrund

    The enhanced specificity Cas9 protein from Streptococcus pyogenes (~160 KD), modified at sites K848A/K1003A/R1060A, exhibit substantially reduced off-target cleavage in cells. When combined with target RNAs, eSpCas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and help the development of genetically-modified animals via one-cell embryo injection.

    Synonyme

    CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9

  • Uniprot ID

    #BDU59491.1

    Sequenznotiz

    Asp41-Asp1407

• Anleitung

  • Rekonstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Versand

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stabilität & Lagerung

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Bild

  SDS-PAGE analysis of recombinant eSpCas9 (1.1).

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Haftungsausschluss：For Research Use or Further Manufacturing Only.