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Diaphorase
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LDG0021RG

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  • Übersicht

    Beschreibung

    Diaphorase, also known as NADH dehydrogenase or NAD(P)H oxidoreductase, is an essential enzyme involved in cellular redox reactions. It facilitates electron transfer from NADH or NADPH to various acceptors, playing a crucial role in the electron transport chain. This enzyme helps mitigate oxidative stress by reducing harmful oxidants and is key in regulating metabolic pathways by maintaining NAD+/NADH and NADP+/NADPH ratios. Additionally, diaphorase contributes to cellular signaling processes, influencing cell proliferation, differentiation, and apoptosis.

  • Spezifikationen

    • Expressionssystem

      Escherichia coli

      Aktivität

      ≥500 U/ mg

    • Einheitsdefinition

      One unit causes decrease in DCPIP by one unit of absorbance (1.0) per minute under the following conditions：27 mM Tris-HCl pH 7.5, 0.2 mM NADH, 40 μM DCPIP and 33 μg/ mL BSA.

      Form

      Lyophilized (Yellow amorphous powder)

  • Anleitung

    • Rekonstitution

      It is recommended to weigh and reconstitute 10 mg of lyophilized powder in 250 μL double-distilled water directly and incubate the solution for at least 10 mins to ensure sufficient re-dissolved.

      Versand

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stabilität & Lagerung

      This product is stable at -20°C for long-term storage under sterile conditions.
      Avoid repeated free-thaw cycles.

  • Bild
    The effects of various chemicals on Diaphorase. The enzyme was incubated with the chemicals at 25°C for 1 hour.

    The effects of various chemicals on Diaphorase.
    The enzyme was incubated with the chemicals at 25°C for 1 hour.

    Temperature activity of Diaphorase. The enzyme reactions in 0.2 M Tris-HCl buffer, pH 7.5, were carried out under different temperature.

    Temperature activity of Diaphorase.
    The enzyme reactions in 0.2 M Tris-HCl buffer, pH 7.5, were carried out under different temperature.

    pH activity of Diaphorase. The buffer conditions with various pH values were used in the reaction at 25°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    pH activity of Diaphorase. The buffer conditions with various pH values were used in the reaction at 25°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    Thermal stability of Diaphorase. The enzyme powder was reconstituted by double-distilled water and treated with different temperature for 30 minutes. Final concentration: 48 U/ mL.

    Thermal stability of Diaphorase. The enzyme powder was reconstituted by double-distilled water and treated with different temperature for 30 minutes. Final concentration: 48 U/ mL.

    pH stability of Diaphorase. The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 3 hours at 30°C. pH 6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0, 0.1 M Carbonate-bicarbonate buffer.

    pH stability of Diaphorase. The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 3 hours at 30°C. pH 6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0, 0.1 M Carbonate-bicarbonate buffer.

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Haftungsausschluss：For Research Use or Further Manufacturing Only.

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