dCas9-VP64

Katalognummer

LDG003POE

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• Spezifikationen

  • Ursprungsart

    Streptococcus pyogenes

    Expressionssystem

    Escherichia coli

  • Affinitäts-Tag

    His Tag (C-term)

    Lagerpuffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Reinheit

    >90% as determined by SDS-PAGE analysis.

    Molekulargewicht

    The protein has a calculated MW of 169.29 kDa.
    The protein migrates as 130-180 kDa under reducing condition (SDS-PAGE analysis).

  • Endotoxingehalt

    <0.1 EU per 1 μg of the protein by the LAL method.

    Form

    Lyophilized

• Hintergrund

  • Hintergrund

    dCas9-VP64 refers to the dCas9 protein (#PDB: 5Y36_A) fused with the VP64 transcription activation domain, which is used in CRISPR-based gene activation. Comprising a deactivated Cas9 (dCas9) protein and VP64 transcriptional activator, it precisely targets gene promoters, enhancing gene expression without altering the DNA sequence. dCas9-VP64 offers a versatile tool for studying gene function, disease mechanisms, and potential therapeutic interventions.
    dCas9 protein is an endonuclease-inactive version of Cas9 (CRISPR associated protein 9). dCas9 lacks endonuclease activity so that it binds but does not cleave cognate DNA, which can be used to localize transcriptional activators or repressors to specific DNA sequences in order to control transcription.

    Synonyme

    CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9

  • Sequenznotiz

    Met1-Asp1368

• Anleitung

  • Rekonstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Versand

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stabilität & Lagerung

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Bild

  SDS-PAGE analysis of recombinant dCas9–VP64.

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Haftungsausschluss：For Research Use or Further Manufacturing Only.