Language
EN
DE
JA
TW
Leadgene Leadgene
Cholesterol Oxidase (CO)
Mehr Bilder
Katalognummer

LDG0025RG

Datenblatt
Weitere Dokumente
Paket & Preis werden geladen...
COA-Suche
  • Übersicht

    Beschreibung

    Cholesterol oxidase (CO) is a bacterial enzyme that catalyzes the oxidation of cholesterol to cholest-4-en-3-one, producing hydrogen peroxide as a byproduct. This enzyme is widely used in clinical diagnostics for measuring cholesterol levels in blood samples, as it plays a crucial role in cholesterol metabolism. Additionally, cholesterol oxidase is used in research to study cholesterol biosynthesis, and in the biotechnology industry for the production of steroids and as a biocatalyst in biosensors. It is primarily sourced from species such as Streptomyces and Brevibacterium.

  • Spezifikationen

    • Expressionssystem

      Escherichia coli

      Aktivität

      ≥40 U/mg

    • Einheitsdefinition

      One unit causes the formation of one micromole of hydrogen peroxide (half a micromole of quinoneimine dye) per minute under the conditions detailed below.
      (87 mM Potassim phosphate buffer, 0.89 mM Cholesterol, 1.4 mM 4-Aminoantipyrine, 21 mM Phenol, 0.34 % Triton X-100, 64 mM Sodium cholate, 33 μg/ mL BSA, 5 U/ mL Peroxidase)

      Form

      Lyophilized (Yellow amorphous powder)

  • Anleitung

    • Rekonstitution

      It is recommended to weight 10 mg of lyophilized powder, reconstitute in 1 mL double-distilled water directly, and incubate the solution for at least 10 mins to ensure sufficient re-dissolved.

      Versand

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stabilität & Lagerung

      This product is stable at -20°C for long-term storage under sterile conditions.
      Avoid repeated free-thaw cycles.

  • Bild
    Temperature activity of Cholesterol oxidase. The enzyme reactions in 0.1 M Potassium phosphate buffer, pH 7.0, were carried out under different temperature

    Temperature activity of Cholesterol oxidase.
    The enzyme reactions in 0.1 M Potassium phosphate buffer, pH 7.0, were carried out under different temperature

    pH activity of Cholesterol oxidase. The buffer conditions with various pH values were used in the reaction at 37°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    pH activity of Cholesterol oxidase.
    The buffer conditions with various pH values were used in the reaction at 37°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    Thermal stability of Cholesterol oxidase. The enzyme powder was reconstituted by double-distilled water and treated with different temperature for 15 minutes. Final concentration: 4.3 U/ mL.

    Thermal stability of Cholesterol oxidase.
    The enzyme powder was reconstituted by double-distilled water and treated with different temperature for 15 minutes. Final concentration: 4.3 U/ mL.

    pH stability of Cholesterol oxidase. The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 20 hours. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    pH stability of Cholesterol oxidase.
    The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 20 hours. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    1
    /
    4
    Gesamt   (4)
  • Bewertung

    Helfen Sie anderen, mehr über dieses Produkt zu erfahren. Nutzen Sie den Link unten, um Ihre Erfahrung zu teilen.

    Bewertung abgeben
  • Publikation

    Derzeit gibt es keine Publikationen. Nutzen Sie den Link unten, um uns zu informieren.

    Publikation einreichen
  • Datenblatt & Dokumente
    Datenblatt
    MSDS
    Handbuch
    1
    /
    2

Haftungsausschluss：For Research Use or Further Manufacturing Only.

Zuletzt aktualisiert：

Diese Website verwendet Cookies wie in unserer Privacy Policy beschrieben, um Ihre Nutzererfahrung zu verbessern und Ihnen maßgeschneiderte Werbung anzubieten. Klicken Sie auf „Ich stimme zu", um zuzustimmen und fortzufahren.