Produktnotiz
Komponenten
2500 U
Item
BspQI (10 U/μL)
Amount
(1 vial) 250 μL
Item
10× R Buffer
Amount
(2 vials) 1.25 mL
Expressionssystem
Escherichia coli
Konzentration
10 U/μL
Lagerpuffer
20 mM Tris-HCl, 500 mM KCl, 0.1 mM EDTA, 1 mM DTT, 500 μg/ml rAlbumin, 50% Glycerol, 0.1% Triton X-100, pH 7
Aktivität
One unit of BspQI is defined as the amount of enzyme that cleave 1 μg λDNA in a total reaction volume of 50 μL at 50°C for 1h.
Endotoxingehalt
<0.1 EU per 1 mL of the enzyme by the LAL method.
Mykoplasmen
Not detected
Form
Liquid
Hintergrund
BspQI is a restriction enzyme derived from bacteria that recognizes and cuts specific DNA sequences. Its recognition sequence is 5'-GCTCTTC-3', with the cleavage site located downstream from the recognition sequence. BspQI is commonly used in molecular biology applications such as genome editing, cloning, and DNA mapping due to its ability to precisely cut DNA at defined sites, facilitating further analysis and manipulation of the DNA molecules.
Versand
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stabilität & Lagerung
This product is stable after storage at:
The enzyme activity of BspQI
In a 50 μL reaction system, 1 μg of λDNA was treated with the 1U/μL of BspQI. The reaction was incubated at 50 °C for 60 minutes, followed by incubation at 80 °C for 20 minutes to inactivate BspQI.
M: DNA marker
Uncut: The control group was not treated with BspQI.
BspQI disgest: The sample group was treated with BspQI.
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Haftungsausschluss：For Research Use or Further Manufacturing Only.
