Leadgene Leadgene
Anti-ZIKV NS1 Antibody [Clone ZK51]
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Katalognummer

LDG0015YA

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  • Übersicht

    Beschreibung

    Zika virus belongs to the family Flaviviridae, genus Flavivirus. Zika virus is transmitted primarily by mosquitos, but also can be transmitted through sexual, vertical, and blood transfusion. NS1 is a highly-conserved viral protein in flaviviruses. In infected patients, the concentration of NS1 protein in circulating blood is relatively high, and can be detected up to 5 days after the onset of illness.

    Produktnotiz

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IFA: 1:500-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Spezifikationen
    • Wirt

      Mouse

      Klonalität

      Monoclonal

    • Isotyp

      IgG1

      Klonname

      clone ZK51

    • Immunogen

      Zika virus NS1

      Reaktivität

      Zika virus

    • Anwendung

      ELISA, WB, IFA

      Konjugation

      Unconjugated

    • Konzentration

      1 mg/mL

      Lagerpuffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Spezifität

      NS1

      Form

      Liquid

  • Anleitung
    • Versand

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stabilität & Lagerung

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Bild
    Immunofluorescence analysis of Anti-ZIKV NS1 Antibody [Clone ZK51] ZIKV NS1 mAb (1:400) were used for detecting ZIKV NS1 in IFA. Scale bar: 200 μm

    Immunofluorescence analysis of Anti-ZIKV NS1 Antibody [Clone ZK51]
    ZIKV NS1 mAb (1:400) were used for detecting ZIKV NS1 in IFA. Scale bar: 200 μm

    ELISA assay of Anti-ZIKV NS1 Antibody [Clone ZK51] Indirect ELISA analysis was performed by coating a plate with recombinant NS1 protein of ZIKV, CHIKV or DENV and probing with anti-ZIKV NS1 Antibody.

    ELISA assay of Anti-ZIKV NS1 Antibody [Clone ZK51]
    Indirect ELISA analysis was performed by coating a plate with recombinant NS1 protein of ZIKV, CHIKV or DENV and probing with anti-ZIKV NS1 Antibody.

    Western blotting analysis of Anti-ZIKV NS1 Antibody [Clone ZK51] Western blot: Virus infection Vero cell lysates were stained with mouse anti-ZIKV NS1 monoclonal antibody at 1:2000 dilution. Lane 1: Vero mock cell lysate (10 μg), Lane 2: Vero ZIKV cell lysate (10 μg), Lane 3: Vero DENV1 cell lysate (10 μg), Lane 4: Vero DENV2 cell lysate (10 μg), Lane 5: Vero DENV3 cell lysate (10 μg), Lane 6: Vero DENV 4 cell lysate (10 μg).

    Western blotting analysis of Anti-ZIKV NS1 Antibody [Clone ZK51]
    Western blot: Virus infection Vero cell lysates were stained with mouse anti-ZIKV NS1 monoclonal antibody at 1:2000 dilution. Lane 1: Vero mock cell lysate (10 μg), Lane 2: Vero ZIKV cell lysate (10 μg), Lane 3: Vero DENV1 cell lysate (10 μg), Lane 4: Vero DENV2 cell lysate (10 μg), Lane 5: Vero DENV3 cell lysate (10 μg), Lane 6: Vero DENV 4 cell lysate (10 μg).

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Haftungsausschluss:For Research Use or Further Manufacturing Only.

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