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LDG0016YA

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Übersicht

Beschreibung

Zika virus belongs to the family Flaviviridae, genus Flavivirus. Zika virus is transmitted primarily by mosquitos, but also can be transmitted through sexual, vertical, and blood transfusion. NS1 is a highly-conserved viral protein in flaviviruses. In infected patients, the concentration of NS1 protein in circulating blood is relatively high, and can be detected up to 5 days after the onset of illness.

Produktnotiz

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:500-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Spezifikationen
- Wirt
  
  Mouse
  
  Klonalität
  
  Monoclonal
- Isotyp
  
  IgG2a
  
  Klonname
  
  clone ZK243
- Immunogen
  
  Zika virus NS1
  
  Reaktivität
  
  Zika virus
- Anwendung
  
  ELISA, WB, IFA, FACS
  
  Konjugation
  
  Unconjugated
- Konzentration
  
  1 mg/mL
  
  Lagerpuffer
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- Spezifität
  
  NS1
  
  Form
  
  Liquid
Anleitung
- Versand
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  Stabilität & Lagerung
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
Bild

ELISA titration of Anti-ZIKV NS1 Antibody [Clone ZK243]
Titration curve of anti-ZIKV NS1 antibody in ELISA. Red: ZIKV NS1; Black: BSA (negative control).

Immunofluorescence analysis of Anti-ZIKV NS1 Antibody [Clone ZK243]
ZIKV NS1 mAb (1:400) were used for detecting ZIKV NS1 in IFA. Scale bar: 200 μm

FACS analysis of Anti-ZIKV NS1 Antibody [Clone ZK243]
ZIKV or DENV-infected Vero cells were stained with mouse anti-ZIKV NS1 monoclonal antibody at 5 μg/mL (red) and without antibody control (black).

ELISA assay of Anti-ZIKV NS1 Antibody [Clone ZK243]
Indirect ELISA analysis was performed by coating a plate with recombinant NS1 protein of ZIKV, CHIKV or DENV and probing with anti-ZIKV NS1 Antibody.

Western blotting analysis of Anti-ZIKV NS1 Antibody [Clone ZK243]
Western blot: Virus infection Vero cell lysates were stained with mouse anti-ZIKV NS1 monoclonal antibody at 1:2000 dilution. Lane 1: Vero mock cell lysate (4 μg), Lane 2: Vero ZIKV cell lysate (4 μg), Lane 3: Vero DENV1 cell lysate (4 μg), Lane 4: Vero DENV2 cell lysate (4 μg), Lane 5: Vero DENV3 cell lysate (4 μg), Lane 6: Vero DENV 4 cell lysate (4 μg).

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