Anti-TGF Beta 1 Antibody [Clone 51-3]

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• Übersicht

  Beschreibung

  Transforming growth factor beta (TGF-β), a polypeptide member of the transforming growth factor beta superfamily of cytokines that includes three isoforms, is involved in various biological processes. TGF-β1, the most abundant TGF-β isoform, is a secreted protein that plays important role in cell proliferation, differentiation and apoptosis and is involved in wound healing, inflammatory processes and prevention of tumor growth.

  Produktnotiz

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-10000
  IFA: 1:400-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Spezifikationen

  • Wirt

    Mouse

    Klonalität

    Monoclonal

  • Isotyp

    IgG1

    Klonname

    clone 51-3

  • Immunogen

    TGF Beta 1

    Reaktivität

    Human

  • Anwendung

    ELISA, WB, IFA, FACS

    Konjugation

    Unconjugated

  • Konzentration

    1 mg/mL

    Lagerpuffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • Spezifität

    TGF Beta 1

    Form

    Liquid

• Anleitung

  • Versand

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stabilität & Lagerung

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Bild

  Immunofluorescence analysis of Anti-TGF Beta 1 Antibody [Clone 51-3]
  293T cells were respectively transfected with TGF beta 1, beta 2 and beta 3
  plasmid and fixed in 4% PFA, permeabilized with PBS containing 0.1%
  Triton X-100. Cells were stained with mouse anti-TGF beta 1 monoclonal
  antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG-
  iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342
  (Blue).

  FACS analysis of Anti-TGF Beta 1 Antibody [Clone 51-3]
  293T cells (black) were respectively transfected with TGF beta 1 (red), beta
  2 (blue) and beta 3 (green) plasmid and stained with mouse anti-TGF beta 1
  monoclonal antibody at 1 μg/ml.

  ELISA titration of Anti-TGF Beta 1 Antibody [Clone 51-3]
  Titration curve of anti-TGF beta 1 antibody in ELISA. Red: TGF beta 1;
  Black: BSA (negative control).

  Western blotting analysis of Anti-TGF Beta 1 Antibody [Clone 51-3]
  Recombinant proteins and TGF beta 1, 2 or 3 transfected 293T cell lysates
  were stained with mouse anti-TGF beta 1 monoclonal antibody at 1:2000
  dilution. Lane 1: recombinant TGF beta 1 protein (25 ng). Lane 2:
  recombinant TGF beta 2 protein (25 ng). Lane 3: recombinant TGF beta 3
  protein (25 ng). Lane 4: TGF beta 1 transfected 293T cell lysates (30 μg).
  Lane 5: TGF beta 2 transfected 293T cell lysates (30 μg). Lane 6: TGF beta
  3 transfected 293T cell lysates (30 μg).

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Haftungsausschluss：For Research Use or Further Manufacturing Only.