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Übersicht

Beschreibung

The TERT antibody targets human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase, which plays a crucial role in maintaining telomere length and cellular immortality. hTERT is highly expressed in most cancer cells, making the hTERT antibody widely used in cancer research and studies related to cellular aging.

Produktnotiz

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:50-500

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Spezifikationen
- Wirt
  
  Mouse
  
  Klonalität
  
  Monoclonal
- Isotyp
  
  IgG1
  
  Klonname
  
  Clone 54-2
- Immunogen
  
  Human TERT Protein
  
  Reaktivität
  
  Human
- Anwendung
  
  ELISA, IFA,WB
  
  Konjugation
  
  Unconjugated
- Konzentration
  
  1 mg/mL
  
  Lagerpuffer
  
  Phosphate Buffered Saline containing 0.03% Proclin300, pH 7.4.
- Form
  
  Liquid
Hintergrund
- Synonyme
  
  Telomerase Reverse Transcriptase (TERT), hTRT, EST2, TP2 (Telomerase-associated Protein 2), Telomerase Catalytic Subunit
Anleitung
- Versand
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  
  Stabilität & Lagerung
  
  This product is stable after storage at:
  ● 2-8°C for 2 weeks under sterile conditions from date of receipt.
  ● -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
Bild

Immunofluorescence: 293T cells were transfected with TERT plasmid and fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with Anti-TERT Antibody [Clone 54-2] (1:50) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with DAPI (Blue).

Western blot: TERT transfected 293T cell lysates were stained with mouse anti-TERT monoclonal antibody.

ELISA: Titration curve of anti-TERT antibody in ELISA. Red: Human TERT; Black: BSA (negative control).

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