Anti-PGA5 Antibody [Clone 85-5]

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• Übersicht

  Beschreibung

  Pepsinogens are members of the peptidases that are secreted by gastric chief cells and are inactive polypeptide proenzymes known as zymogens. They are converted by gastric acid to active pepsins, which are involved in the digestion of proteins. Pepsinogen A5 (PGA5), an isozymogen of pepsinogen A, is the predominant endopeptidase in the gastric acid and is associated with atrophic gastritis and gastritis.

  Produktnotiz

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IFA: 1:100-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Spezifikationen

  • Wirt

    Mouse

    Klonalität

    Monoclonal

  • Isotyp

    IgG1

    Klonname

    clone 85-5

  • Immunogen

    The immunogen information used to generate this antibody is proprietary.

    Reaktivität

    Human

  • Anwendung

    ELISA, WB, IFA, FACS

    Konjugation

    Unconjugated

  • Konzentration

    1 mg/mL

    Lagerpuffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • Spezifität

    PGA5

    Form

    Liquid

• Anleitung

  • Versand

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stabilität & Lagerung

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Bild

  Immunofluorescence analysis of Anti-PGA5 Antibody [Clone 85-5]
  HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
  Triton X-100. Cells were stained with mouse anti-PGA5 monoclonal antibody
  (1:100) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
  1:200, green) and cell nuclei were stained with Hoechst 33342 (Blue).

  FACS analysis of Anti-PGA5 Antibody [Clone 85-5]
  K562 cells were stained with mouse anti-PGA5 monoclonal antibody at 2
  μg/ml (red) and without antibody control (black).

  ELISA analysis of Anti-PGA5 Antibody [Clone 85-5]
  The specificity of anti-PGA5 antibody recognize recombinant proteins in
  ELISA. Red: Porcine pepsin; Orange: PGA5; Green: Pepsinogen I; Blue:
  Pepsinogen II; Black: BSA (negative control).

  Western blotting analysis of Anti-PGA5 Antibody [Clone 85-5]
  Recombinant proteins and mouse stomach lysates were stained with mouse
  anti-PGA5 monoclonal antibody at 1:5000 dilution. Lane 1: Purified human
  pepsinogen I (25 ng). Lane 2: Purified human pepsinogen II (25 ng). Lane 3:
  Mouse stomach lysate (30 μg). Lane 4: Human gastric juice (10 μL).

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• Datenblatt & Dokumente

  Datenblatt

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Haftungsausschluss：For Research Use or Further Manufacturing Only.