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Leadgene Leadgene
Anti-IL-2 Antibody [Clone 62-2]
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LDG0022YA

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  • Übersicht

    Beschreibung

    The gamma-chain (γ-chain) cytokines regulate the development, survival, proliferation, differentiation and function of cells of the immune system. Interleukin-2 (IL-2), a member of the γ-chain cytokines, is a cytokine produced by helper T cells. This cytokine has immunostimulatory and immunoregulatory functions and plays an important role in induction of immunity through proliferation and activation of T cells, stimulation of NK cells, and homeostasis.

    Produktnotiz

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IFA: 1:400-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Spezifikationen

    • Wirt

      Mouse

      Klonalität

      Monoclonal

    • Isotyp

      IgG1

      Klonname

      clone 62-2

    • Immunogen

      Interleukin 2

      Reaktivität

      Human

    • Anwendung

      ELISA, WB, IFA, FACS

      Konjugation

      Unconjugated

    • Konzentration

      1 mg/mL

      Lagerpuffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Spezifität

      Interleukin 2

      Form

      Liquid

  • Anleitung

    • Versand

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stabilität & Lagerung

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Bild
    Immunofluorescence analysis of Anti-IL-2 Antibody [Clone 62-2] MCF-7 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-IL-2 monoclonal antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescence analysis of Anti-IL-2 Antibody [Clone 62-2]
    MCF-7 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
    Triton X-100. Cells were stained with mouse anti-IL-2 monoclonal antibody
    (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
    1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    FACS analysis of Anti-IL-2 Antibody [Clone 62-2] MCF-7 cells were stained with mouse anti-IL-2 monoclonal antibody at 1 μg/ml (red) and without antibody control (black).

    FACS analysis of Anti-IL-2 Antibody [Clone 62-2]
    MCF-7 cells were stained with mouse anti-IL-2 monoclonal antibody at 1
    μg/ml (red) and without antibody control (black).

    ELISA titration of Anti-IL-2 Antibody [Clone 62-2] Titration curve of anti-IL-2 antibody in ELISA. Red: IL-2; Black: BSA (negative control).

    ELISA titration of Anti-IL-2 Antibody [Clone 62-2]
    Titration curve of anti-IL-2 antibody in ELISA. Red: IL-2; Black: BSA (negative control).

    Western blotting analysis of Anti-IL-2 Antibody [Clone 62-2] Recombinant proteins and MCF-7 cell lysates were stained with mouse anti- IL-2 monoclonal antibody at 1:2000 dilution. Lane 1: recombinant IL-2 protein (100 ng). Lane 2: MCF-7 cell lysates (60 μg).

    Western blotting analysis of Anti-IL-2 Antibody [Clone 62-2]
    Recombinant proteins and MCF-7 cell lysates were stained with mouse anti-
    IL-2 monoclonal antibody at 1:2000 dilution. Lane 1: recombinant IL-2 protein
    (100 ng). Lane 2: MCF-7 cell lysates (60 μg).

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Haftungsausschluss：For Research Use or Further Manufacturing Only.

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