Anti-GIF Antibody [Clone 50-1]

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• Übersicht

  Beschreibung

  Metallothioneins (MTs) is a group of low-molecular-weight, cysteine-rich, non-enzymatic proteins, is involved in storage, transportation and binding of metals, and plays a critical role in the protection against metal toxicity and oxidative stress. Metallothionein-3 (MT-3；also known as Growth Inhibitory Factor, GIF), a member of the MTs family, is mainly expressed in the central nervous system, can protect neuronal cells from oxidative stress via its anti-oxidant properties. MT-3 is abnormally under-expressed in the brains of Alzheimer's disease patients.

  Produktnotiz

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-10000
  IFA: 1:100-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Spezifikationen

  • Wirt

    Mouse

    Klonalität

    Monoclonal

  • Isotyp

    IgG1

    Klonname

    clone 50-1

  • Immunogen

    GIF

    Reaktivität

    Human

  • Anwendung

    ELISA, WB, IFA, FACS

    Konjugation

    Unconjugated

  • Konzentration

    1 mg/mL

    Lagerpuffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • Spezifität

    GIF

    Form

    Liquid

• Anleitung

  • Versand

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stabilität & Lagerung

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Bild

  Immunofluorescence analysis of Anti-GIF Antibody [Clone 50-1]
  HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
  Triton X-100. Cells were stained with mouse anti-GIF monoclonal antibody
  (1:100) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
  1:200, green) and cell nuclei were stained with Hoechst 33342 (Blue).

  FACS analysis of Anti-GIF Antibody [Clone 50-1]
  K562 cells were stained with mouse anti-GIF monoclonal antibody at 2 μg/ml
  (red) and without antibody control (black).

  ELISA titration of Anti-GIF Antibody [Clone 50-1]
  Titration curve of anti-GIF antibody in ELISA. Red: GIF; Black: BSA (negative
  control).

  Western blotting analysis of Anti-GIF Antibody [Clone 50-1]
  Recombinant proteins and mouse stomach lysates were stained with mouse
  anti-GIF monoclonal antibody at 1:5000 dilution. Lane 1: Recombinant GIF
  protein (100 ng). Lane 2: Mouse stomach lysate (30 μg). Lane 3: Human
  gastric juice (10 μL).

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• Datenblatt & Dokumente

  Datenblatt

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  Handbuch

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Haftungsausschluss：For Research Use or Further Manufacturing Only.