Anti-CXCL12 Antibody [Clone 69-2]

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• Übersicht

  Beschreibung

  The CXC chemokine is a group of secreted proteins, attracts neutrophils and lymphocytes. CXC chemokine ligand 12 (CXCL12 or Stromal cell-derived factor 1, SDF-1), a member of the CXC chemokines, exists in three alternative splicing variants (α, β and γ). This chemokine plays a crucial role in embryogenesis, hematopoiesis, angiogenesis and inflammation. CXCL12 is the only chemokine ligand for CXCR4, this ligand-receptor pair is essential during embryogenesis, hematopoiesis, and immune system organization.

  Produktnotiz

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-10000
  IFA: 1:200-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Spezifikationen

  • Wirt

    Mouse

    Klonalität

    Monoclonal

  • Isotyp

    IgG2a

    Klonname

    clone 69-2

  • Immunogen

    CXCL12

    Reaktivität

    Human

  • Anwendung

    ELISA, WB, IFA, FACS

    Konjugation

    Unconjugated

  • Konzentration

    1 mg/mL

    Lagerpuffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • Spezifität

    CXCL12

    Form

    Liquid

• Anleitung

  • Versand

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stabilität & Lagerung

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Bild

  Immunofluorescence analysis of Anti-CXCL12 Antibody [Clone 69-2]
  A549 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
  Triton X-100. Cells were stained with mouse anti-CXCL12 monoclonal
  
  antibody (1:200) followed by secondary antibodies (goat anti-Mouse IgG-
  iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342
  
  (Blue).

  FACS analysis of Anti-CXCL12 Antibody [Clone 69-2]
  A549 cells were stained with mouse anti-CXCL12 monoclonal antibody at 2
  μg/ml (red) and without antibody control (black).

  ELISA titration of Anti-CXCL12 Antibody [Clone 69-2]
  Titration curve of anti-CXCL12 antibody in ELISA. Red: CXCL12; Black: BSA
  (negative control).

  Western blotting analysis of Anti-CXCL12 Antibody [Clone 69-2]
  HeLa cell lysates (30 μg) were stained with mouse anti-CXCL12 monoclonal
  antibody at 1:5000 dilution.

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• Datenblatt & Dokumente

  Datenblatt

  MSDS

  Handbuch

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Haftungsausschluss：For Research Use or Further Manufacturing Only.