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Leadgene Leadgene
Anti-CD326 Antibody [Clone 1H7]
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LDG0070YA

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  • Übersicht

    Beschreibung

    Mouse anti-human CD326 mAb recognizes CD326, also known as Epithelial Cell Adhesion Molecule (EpCAM). CD326 expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.

    Produktnotiz

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IFA: 1:500-1000
    FACS: 1:500-1000

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Spezifikationen

    • Wirt

      Mouse

      Klonalität

      Monoclonal

    • Isotyp

      IgG2a

      Klonname

      clone 1H7

    • Immunogen

      MDA-MB-231 adenocarcinoma cell line

      Reaktivität

      Human

    • Anwendung

      ELISA, WB, IFA, FACS

      Konjugation

      Unconjugated

    • Konzentration

      1 mg/mL

      Lagerpuffer

      Phosphate Buffered Saline, pH 7.4.

    • Spezifität

      CD326

      Form

      Liquid

  • Anleitung

    • Versand

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stabilität & Lagerung

      This product is stable after storage at:

      • 2-8°C for 6 months under sterile conditions from date of receipt.

      Briefly centrifuge vials before opening.

  • Bild
    Immunofluorescence analysis of anti-CD326 mAb SW480 cells were fixed in 4% PFA. Experimental cells were stained with mouse anti-human CD326 monoclonal antibody (1:500) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green). All cell nuclei were stained with Hoechst 33342 (blue).

    Immunofluorescence analysis of anti-CD326 mAb
    SW480 cells were fixed in 4% PFA. Experimental cells were stained with
    mouse anti-human CD326 monoclonal antibody (1:500) followed by
    secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green). All cell
    nuclei were stained with Hoechst 33342 (blue).

    Flow Cytometry analysis of anti-CD326 mAb MDA-MB-231 and Jurkat cells were stained with mouse anti-human CD326 monoclonal antibody at 1 μg/ml (red) and without antibody control (black).

    Flow Cytometry analysis of anti-CD326 mAb
    MDA-MB-231 and Jurkat cells were stained with mouse anti-human CD326
    monoclonal antibody at 1 μg/ml (red) and without antibody control (black).

    Western blotting analysis of anti-CD326 mAb (1:1000) SW480 cell lysate (non-reduced) (30 μg)

    Western blotting analysis of anti-CD326 mAb (1:1000)
    SW480 cell lysate (non-reduced) (30 μg)

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Haftungsausschluss：For Research Use or Further Manufacturing Only.

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