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Leadgene Leadgene
Anti-c-Met Antibody [Clone 4A9]
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LDG0045YA

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  • Übersicht

    Beschreibung

    The c-mesenchymal epithelial transition factor (c-Met；also known as hepatocyte growth factor receptor, HGFR), is a receptor tyrosine kinase (RTK) that mainly exists in epithelial cells. c-Met and its high-affinity ligand, hepatocyte growth factor (HGF), play important roles in mediating embryogenesis, tissue regeneration, wound healing and the formation of nerve and muscle. Aberrant HGF/c-Met axis activation is associated with the proliferation, survival, invasion and metastasis of various tumor cells, and thus c-Met may be a tumor biomarker and therapeutic target.

    Produktnotiz

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-10000
    IFA: 1:200-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Spezifikationen

    • Wirt

      Mouse

      Klonalität

      Monoclonal

    • Isotyp

      IgG1

      Klonname

      clone 4A9

    • Immunogen

      c-Met

      Reaktivität

      Human

    • Anwendung

      ELISA, WB, IFA, FACS

      Konjugation

      Unconjugated

    • Konzentration

      1 mg/mL

      Lagerpuffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Spezifität

      c-Met

      Form

      Liquid

  • Anleitung

    • Versand

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stabilität & Lagerung

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Bild
    Immunofluorescence analysis of Anti-c-Met Antibody [Clone 4A9] MCF7 cells were fixed in 100% methanol, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-c-Met monoclonal antibody (1:200) followed by secondary antibodies (goat anti-Mouse IgG- iFluor 488, 1:200, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescence analysis of Anti-c-Met Antibody [Clone 4A9]
    MCF7 cells were fixed in 100% methanol, permeabilized with PBS containing
    0.1% Triton X-100. Cells were stained with mouse anti-c-Met monoclonal
    antibody (1:200) followed by secondary antibodies (goat anti-Mouse IgG-
    iFluor 488, 1:200, green) and cell nuclei were stained with Hoechst 33342
    (Blue).

    FACS analysis of Anti-c-Met Antibody [Clone 4A9] MCF-7 cells were stained with anti-c-Met monoclonal antibody at 2 μg/ml (red) and without antibody control (black).

    FACS analysis of Anti-c-Met Antibody [Clone 4A9]
    MCF-7 cells were stained with anti-c-Met monoclonal antibody at 2 μg/ml
    (red) and without antibody control (black).

    ELISA titration of Anti-c-Met Antibody [Clone 4A9] Titration curve of anti-c-Met antibody in ELISA. Red: c-Met; Black: BSA (negative control).

    ELISA titration of Anti-c-Met Antibody [Clone 4A9]
    Titration curve of anti-c-Met antibody in ELISA. Red: c-Met; Black: BSA
    (negative control).

    Western blotting analysis of Anti-c-Met Antibody [Clone 4A9] HeLa cell lysates (50 μg) were stained with mouse anti-c-Met monoclonal antibody at 1:5000 dilution.

    Western blotting analysis of Anti-c-Met Antibody [Clone 4A9]
    HeLa cell lysates (50 μg) were stained with mouse anti-c-Met monoclonal
    antibody at 1:5000 dilution.

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  • Datenblatt & Dokumente
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Haftungsausschluss：For Research Use or Further Manufacturing Only.

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