Leadgene Leadgene
Alpha-2,3-Sialidase
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LDG0024RG

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  • Übersicht

    Beschreibung

    Alpha-2,3-sialidase is an enzyme that specifically cleaves sialic acid residues linked to glycoproteins or glycolipids via an α-2,3 linkage. This glycosidase plays a crucial role in the modification and degradation of sialic acids, which are important for various biological processes, including cell signaling, immune response, and pathogen recognition. Alpha-2,3-sialidase activity is essential in studying viral infections, such as influenza, where the removal of sialic acid residues can affect viral binding and entry into host cells.

  • Spezifikationen
    • Expressionssystem

      Escherichia coli

      Konzentration

      50 U/μL

    • Lagerpuffer

      20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5

      Reinheit

      >95% as determined by SDS-PAGE analysis.

    • Einheitsdefinition

      One unit is defined as the enzyme required to cleave > 95% of the terminal α-Neu5Ac from 1 nanomole Neu5Ac-GalGalNAc of glycoprotein in 1 hour at 37°C in 40 μL reaction buffer (50 mM Tris-HCl, 100 mM NaCl, pH 7.5).

      Endotoxingehalt

      <1 EU per 1 μg of the protein by the LAL method

    • Form

      Liquid

  • Anleitung
    • Versand

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stabilität & Lagerung

      This product is stable after storage at:

      • -20°C for -80°C long-term storage under sterile conditions.

      Avoid repeated free-thaw cycles.

  • Bild
    The standard assay was performed by incubating 1 unit of alpha-2,3-sialidase and 1 nanomole of Fetuin under the above conditions. SDS-PAGE analysis of Fetuin digested with alpha-2,3-sialidase.

    The standard assay was performed by incubating 1 unit of alpha-2,3-sialidase and 1 nanomole of Fetuin under the above conditions.
    SDS-PAGE analysis of Fetuin digested with alpha-2,3-sialidase.

    SDS-PAGE analysis of recombinant alpha-2,3-sialidase.

    SDS-PAGE analysis of recombinant alpha-2,3-sialidase.

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  • Datenblatt & Dokumente

Haftungsausschluss:For Research Use or Further Manufacturing Only.

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