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Leadgene Leadgene
Anti-GST Tag Antibody Sepharose Purification Kit
Catalog Number

LDG0008RD

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  • Overview

    Description

    Affinity purified anti-GST antibodies, developed in mouse, were conjugated to NHS-sepharose. It is an efficient technique for isolating recombinant proteins or other proteins. The GST epitope system relies on a total 211 amino acids long recombinant antibody, which is able to react with N- and C- terminal GST-tagged fusion proteins and may be used for the immunoprecipitation or immune affinity purification.
    The purified antibody is immobilized at 3-5 mg antibody per mL of 50% slurry and this kit allows a rapid and efficient affinity purification of active GST fusion proteins. The affinity resin allows an efficient binding of GST fusion proteins without the need for preliminary steps and calibrations. The affinity bound GST fusion proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.

    Product Note

    The optimal protein concentration of the cell lysate should be determined by the end user.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Application

      Immunoprecipitation, Immunoaffinity Purification

      Conjugation

      Sepharose

    • Specificity

      Recognizes GST tag on either the N- or C-terminus of the recombinant fusion protein.

      Sample Type

      Cell lysate

    • Binding capacity

      The binding and elution capacity of 1 mL Mouse anti-GST tag mAb sepharose are commonly more than 1 mg of GST fusion proteins.

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product

      Please refer to product manual for storage constructions

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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