SARS-CoV-2 Spike Protein (S1 Subunit), His Tag, E. coli

Catalog Number

LDG005PVE

Datasheet

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• Specifications

  • Species of Origin

    SARS-CoV-2

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 74.34 kDa.
    The protein migrates as 63-75 kDa under reducing condition (SDS-PAGE analysis).

  • Form

    Lyophilized

• Background

  • Background

    SARS‑CoV‑2 is a kind of coronavirus which full name is severe acute respiratory syndrome coronavirus 2. SARS‑CoV‑2 is contagious that causes the respiratory diseases and lung diseases which make difficulty breathing. SARS‑CoV‑2 do the spillover event in 2019 because it has genetic diversity. SARS-CoV-2 is composed by four subunits (spike, envelope, membrane and nucleocapsid proteins). Its RNA genome is encapsulated with nucleocapsid protein. The viral envelope is comprised of spike, envelope and membrane protein. SARS‑CoV‑2 has high affinity to ACE2, which is highly expression in intestines, kidney, and heart.

    Synonyms

    Spike glycoprotein, S glycoprotein, E2, Peplomer protein

  • Uniprot ID

    #P0DTC2

    Sequence Note

    Val16-Cys671

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant SARS-CoV-2 spike protein (S1 subunit).

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• Publication

  1. Wu CC et al. Biosensors (Basel). 2022; 12(5):265.
  2. Chen PH et al. Sens Actuators B Chem. 2022; 357:131415.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.