Language
TW
Leadgene Leadgene
Anti-V5 Tag Antibody [Clone 7-1-8]
Catalog Number

LDG0003YB

More Documents
Loading package & price...
  • Overview

    Description

    Mouse monoclonal anti-V5 antibody is an antibody specific to GKPIPNPLLGLDST (V5) tags which can be applied to several immunoassays including ELISA, WB, IHC, and Flow cytometry.
    This antibody has been identified in several sources (bacteria, yeast, and mammalian cells) of V5-fusion protein.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IP: 1:500-2000
    IFA: 1:500-1000
    FACS: 1:500-1000

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG2b

      Clone Name

      clone 7-1-8

    • Immunogen

      Pepetide: GKPIPNPLLGLDST (V5)

      Application

      ELISA, WB, IP, IFA, FACS

    • Conjugation

      Unconjugated

      Concentration

      1 mg/mL

    • Storage Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

      Specificity

      Recognizes tag sequence of GKPIPNPLLGLDST on either the N- or C-terminus of recombinant fusion protein.

    • Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Western blotting analysis of anti-V5 tag mAb (1:5000) Lane 1: 293T whole cell lysate-untransfected Lane 2: 293T whole cell lysate-transfected Lysate/proteins at 20 μg per lane

    Western blotting analysis of anti-V5 tag mAb (1:5000)
    Lane 1: 293T whole cell lysate-untransfected
    Lane 2: 293T whole cell lysate-transfected
    Lysate/proteins at 20 μg per lane

    1
    /
    1
    Total   (1)
  • Review

    Help others learn more about this product. Use the link below to share your experience.

  • Publication

    There are currently no publications. Use the link below to let us know.

  • Datasheet & Documents
    MSDS
    Manual
    1
    /
    1

Disclaimer:For Research Use or Further Manufacturing Only.

This site uses cookies as described in our Privacy Policy in order to improve your user experience and deliver customized advertising to you. Click “I Agree” to consent and continue.