Anti-MMP7 Antibody [Clone 20-9]

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Catalog Number

LDG0043YA

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• Overview

  Description

  Matrix metalloproteinases (MMPs) is a group of proteolytic enzymes that targets many extracellular proteins including other proteases, growth factors, cell surface receptors and adhesion molecules, plays an important role in many physiological and pathological processes. Matrix metalloproteinase-7 (MMP-7；matrilysin-1；PUMP-1), a member of the MMP family, can degrade a large series of proteins of the extracellular matrix and other substrates. MMP-7 is an important factor for normal tissue remodeling and wound healing and is associated with the occurrence and development of various tumors, thus may be a tumor biomarker and therapeutic target.

  Product Note

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-10000
  IFA: 1:200-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG2a

    Clone Name

    clone 20-9

  • Immunogen

    MMP7

    Reactivity

    Human

  • Application

    ELISA, WB, IFA, FACS

    Conjugation

    Unconjugated

  • Concentration

    1 mg/mL

    Storage Buffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • Specificity

    Recognizes mature MMP7, do no cross-reactivity with pro-MMP7

    Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Image

  Immunofluorescence analysis of Anti-MMP7 Antibody [Clone 20-9]
  A549 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
  Triton X-100. Cells were stained with mouse anti-MMP7 monoclonal antibody
  (1:200) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
  1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

  FACS analysis of Anti-MMP7 Antibody [Clone 20-9]
  A549 cells were stained with mouse anti-MMP7 monoclonal antibody at 2
  μg/ml (red) and without antibody control (black).

  ELISA titration of Anti-MMP7 Antibody [Clone 20-9]
  Titration curve of anti-MMP7 antibody in ELISA. Red: MMP7 (active); Green:
  MMP7 (proenzyme); Black: BSA (negative control).

  Western blotting analysis of Anti-MMP7 Antibody [Clone 20-9]
  Recombinant proteins and LPS treat MMP7 transfected 293T cell lysates
  were stained with mouse anti-MMP7 (20-9) monoclonal antibody at 1:5000
  dilution. Lane 1: recombinant MMP7 (proenzyme) protein (50 ng). Lane 2:
  recombinant MMP7 (active) protein (50 ng). Lane 3: 293T cell lysates (30
  μg). Lane 4: lysates from LPS treat 293T cells (30 μg). Lane 5: MMP7
  transfected 293T cell lysates (30 μg). Lane 6: lysates from LPS treat MMP7
  transfected 293T cell lysates (30 μg). The expression of Actin was as the
  internal control.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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