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Leadgene Leadgene
Anti-RAGE Antibody [Clone 16-1]
Catalog Number

LDG0039YA

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  • Overview

    Description

    The immunoglobulin superfamily is a group of transmembrane proteins, contains a variable number of disulfide-stabilized motifs similar to immunoglobulins, associated with the adhesion, binding and recognition processes of cells. Receptor for advanced glycation endproducts (RAGE), a member of the immunoglobulin superfamily, not only binds to advanced glycation endproducts but also interacts with multiple ligands having common motifs. These interactions play important roles in various cellular processes, and associated with many human diseases.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-10000
    IFA: 1:200-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG1

      Clone Name

      clone 16-1

    • Immunogen

      RAGE

      Reactivity

      Human

    • Application

      ELISA, WB, IFA, FACS

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Storage Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      RAGE

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Immunofluorescence analysis of Anti-RAGE Antibody [Clone 16-1] HEK293T cells were transfected with RAGE plasmid and fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-RAGE monoclonal antibody (1:200) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:200, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescence analysis of Anti-RAGE Antibody [Clone 16-1]
    HEK293T cells were transfected with RAGE plasmid and fixed in 4% PFA,
    permeabilized with PBS containing 0.1% Triton X-100. Cells were stained
    with mouse anti-RAGE monoclonal antibody (1:200) followed by secondary
    antibodies (goat anti-Mouse IgG-iFluor 488, 1:200, green) and cell nuclei
    were stained with Hoechst 33342 (Blue).

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  • Datasheet & Documents
    MSDS
    Manual
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