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Leadgene Leadgene
Anti-DENV NS3 Antibody [Clone 15-5]
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Catalog Number

LDG0006YA

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  • Overview

    Description

    Dengue virus belongs to the family Flaviviridae, genus Flavivirus and consists of four distinct serotypes (DENV1 to 4). Dengue virus is transmitted by mosquitos majorly of species Aedes aegypti and Aedes Albopictus that widespread around tropics and subtropics. NS3 is a multi-functional enzyme, acting as a protease for polyprotein processing. The NS3 proteins are essential for viral replication and infectivity and important targets for anti-dengue drug design.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IFA: 1:500-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG2b

      Clone Name

      clone 15-5

    • Immunogen

      Dengue virus NS3

      Reactivity

      Dengue virus

    • Application

      ELISA, WB, IFA, FACS

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Storage Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      NS3

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    ELISA titration of DENV NS3 antibody

    ELISA titration of DENV NS3 antibody

    Western blotting analysis of DENV NS3 antibody (1:1000) Lane 1: DENV1-infected Vero cell lysate Lane 2: DENV2-infected Vero cell lysate Lane 3: DENV3-infected Vero cell lysate Lane 4: DENV4-infected Vero cell lysate Lane 5: ZIKV-infected Vero cell lysate

    Western blotting analysis of DENV NS3 antibody (1:1000)
    Lane 1: DENV1-infected Vero cell lysate
    Lane 2: DENV2-infected Vero cell lysate
    Lane 3: DENV3-infected Vero cell lysate
    Lane 4: DENV4-infected Vero cell lysate
    Lane 5: ZIKV-infected Vero cell lysate

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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