LeadGMP® Human TGF Beta 1 Protein, His Tag, E. coli

Catalog Number

LDG015PHE-GMP

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 13.7 kDa.
    The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to inhibit the IL-4 dependent proliferation in TF-1 cells. The ED₅₀ for this effect is <0.1 ng/mL. The specific activity of recombinant human TGF beat 1 is approximately > 1 x 10⁷ IU/mg, which is calibrated against the human TGF Beta 1 Non WHO Reference Material (NIBSC code: 89/514).

    Endotoxin Level

    <0.05 EU per 1 µg of the protein by the LAL method.

  • Mycoplasma

    Not detected

    Form

    Lyophilized

• Background

  • Background

    The transforming growth factor beta (TGF beta) family of cytokines are ubiquitous, multifunctional, and essential to survival. They play the central roles in growth, development, inflammation, repair, and host immunity. The mammalian TGF beta isoforms (TGF beta 1, beta 2 and beta 3) are secreted as potential precursors and possess a variety of cell surface receptors and produces at least two mediate signal transductions.

    Synonyms

    Transforming growth factor beta-1 proprotein

  • Uniprot ID

    P01137

    Sequence Note

    Ala279-Ser390

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. In some experiments, it recommends to add 10 mM HCl when reconstitute lyophilized protein.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

    Manufacturing Specifications

    LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

    • Animal-free reagent and laboratory
    • Manufactured and tested under GMP guideline
    • Testing and traceability of raw material
    • Records of the maintenance and equipment calibration
    • Personnel training records
    • Batch-to-batch consistency
    • Documentation of QA control and process changes
    • Manufactured and tested under an ISO 13485:2016 certified quality management system
    • Stability monitor of product shelf-life

• Image

  SDS-PAGE analysis of LeadGMP® TGF beta 1, Human

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.