Mouse Flt-3 Ligand, His Tag, E. coli

Catalog Number

LDG073PME

Datasheet

More Documents

Loading package & price...

COA Search

• Specifications

  • Species of Origin

    Mouse

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 19.3 kDa.
    The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce proliferation in BaF3 cells transfected with mouse Flt-3. The ED₅₀ for this effect is <2 ng/mL.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Fms-related tyrosine kinase-3 ligand (Flt-3 Ligand) is a protein which is encoded by the Flt3lg gene in mouse. As an important regulator of hematopoiesis, Flt-3 ligand has a tyrosine-protein kinase activity that stimulate the proliferation of hematopoietic progenitor cells of both lymphoid and myeloid origin. Flt-3 ligand can synergistically increase the proliferation of immature progenitors with other cytokines such as GM-CSF, G-CSF, IL-3, EPO, IL-11, IL-12, IL-6 or TPO. Flt-3 ligand is expressed by a variety of hematopoietic progenitor cells including immature hematopoietic and marrow stromal cells.

    Synonyms

    Fms-related tyrosine kinase 3 ligand, Flt3L, SL cytokine

  • Uniprot ID

    #P49772

    Sequence Note

    Gly27-Arg188

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant mouse Flt-3 Ligand.

  1

  /

  1

  Total   (1)

• Review

  

  Help others learn more about this product. Use the link below to share your experience.

  Submit a Review

• Publication

  

  There are currently no publications. Use the link below to let us know.

  Submit a Publication

• Datasheet & Documents

  Datasheet

  MSDS

  Manual

  1

  /

  1

Disclaimer：For Research Use or Further Manufacturing Only.