Anti-IL-38 Antibody [Clone 56-1]

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Catalog Number

LDG0034YA

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• Overview

  Description

  Interleukin-38 (IL-38, or IL-1F10), a member of the IL-1 family, is a cytokine with anti-inflammatory properties, widely expressed in the placenta, tonsil B lymphocytes, spleen, skin and thymus. IL-38 has homology to IL-1 receptor antagonist (IL-1Ra) and IL-36 receptor antagonist (IL-36Ra), thus it can bind to several receptors to inhibit production of inflammatory cytokines, plays an important role in the regulation of inflammation and immune responses.

  Product Note

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IFA: 1:200-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG2b

    Clone Name

    clone 56-1

  • Immunogen

    Interleukin 38

    Reactivity

    Human

  • Application

    ELISA, WB, IFA, FACS

    Conjugation

    Unconjugated

  • Concentration

    1 mg/mL

    Storage Buffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • Specificity

    Interleukin 38

    Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Image

  Immunofluorescence analysis of Anti-IL-38 Antibody [Clone 56-1]
  A549 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
  Triton X-100. Cells were stained with mouse anti-IL-38 monoclonal antibody
  (1:200) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
  1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

  FACS analysis of Anti-IL-38 Antibody [Clone 56-1]
  A549 cells were stained with mouse anti-IL-38 monoclonal antibody at 2
  μg/ml (red) and without antibody control (black).

  ELISA titration of Anti-IL-38 Antibody [Clone 56-1]
  Titration curve of anti-IL-38 antibody in ELISA. Red: IL-38; Black: BSA
  (negative control).

  Western blotting analysis of Anti-IL-38 Antibody [Clone 56-1]
  Recombinant IL-38 protein and IL-38 transfected 293T cell lysates were
  stained with mouse anti-IL-38 monoclonal antibody at 1:5000 dilution. Lane
  1: Recombinant IL-38 protein (50 ng). Lane 2: 293T cell lysates (30 μg). Lane
  3: IL-38 transfected 293T cell lysates (30 μg).

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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